Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [3]
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Validation data
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- Product number
- NBP1-84725 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-84725, RRID:AB_11025931
- Product name
- Rabbit Polyclonal Fibulin 1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human Fibulin 1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Affinity proteomics reveals elevated muscle proteins in plasma of children with cerebral malaria.
Plasma profiling reveals human fibulin-1 as candidate marker for renal impairment.
Promoter hypermethylation of fibulin 1 gene is associated with tumor progression in hepatocellular carcinoma.
Bachmann J, Burté F, Pramana S, Conte I, Brown BJ, Orimadegun AE, Ajetunmobi WA, Afolabi NK, Akinkunmi F, Omokhodion S, Akinbami FO, Shokunbi WA, Kampf C, Pawitan Y, Uhlén M, Sodeinde O, Schwenk JM, Wahlgren M, Fernandez-Reyes D, Nilsson P
PLoS pathogens 2014 Apr;10(4):e1004038
PLoS pathogens 2014 Apr;10(4):e1004038
Plasma profiling reveals human fibulin-1 as candidate marker for renal impairment.
Neiman M, Hedberg JJ, Dönnes PR, Schuppe-Koistinen I, Hanschke S, Schindler R, Uhlén M, Schwenk JM, Nilsson P
Journal of proteome research 2011 Nov 4;10(11):4925-34
Journal of proteome research 2011 Nov 4;10(11):4925-34
Promoter hypermethylation of fibulin 1 gene is associated with tumor progression in hepatocellular carcinoma.
Kanda M, Nomoto S, Okamura Y, Hayashi M, Hishida M, Fujii T, Nishikawa Y, Sugimoto H, Takeda S, Nakao A
Molecular carcinogenesis 2011 Aug;50(8):571-9
Molecular carcinogenesis 2011 Aug;50(8):571-9
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Fibulin 1 Antibody [NBP1-84725] - Analysis in human cell lines HeLa and SK-MEL-30 using Anti-FBLN1 antibody. Corresponding FBLN1 RNA-seq data are presented for the same cell lines. Loading control: Anti-COX4I1.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Fibulin 1 Antibody [NBP1-84725] - Analysis in control (vector only transfected HEK293T lysate) and FBLN1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Fibulin 1 Antibody [NBP1-84725] - Staining of human cervix, uterine shows high expression.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Fibulin 1 Antibody [NBP1-84725] - Staining of human liver shows low expression as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Fibulin 1 Antibody [NBP1-84725] - Staining in human cervix, uterine and liver tissues using anti-FBLN1 antibody. Corresponding FBLN1 RNA-seq data are presented for the same tissues.