H00051176-M03

antibody from Abnova Corporation

Targeting: LEF1 TCF10, TCF1ALPHA, TCF7L3

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00051176-M03

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00051176-M03, RRID:AB_606503

Product name

LEF1 monoclonal antibody (M03), clone 5A3

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant LEF1.

Antigen sequence

DPELCATDEMIPFKDEGDPQKEKIFAEISHPEEEG
DLADIKSSLVNESEIIPASNGHEVARQAQTSQEPY
HDKAREHPDDGKHPDGGLYNKGPSYSSYSGYIMMP
NMNND


Isotype

IgG

Antibody clone number

5A3

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

LEF1 monoclonal antibody (M03), clone 5A3 Western Blot analysis of LEF1 expression in Jurkat ( Cat # L017V1 ).

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

LEF1 monoclonal antibody (M03), clone 5A3. Western Blot analysis of LEF1 expression in HepG2 ( Cat # L019V1 ).

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged LEF1 is approximately 0.3ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to LEF1 on HeLa cell. [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol