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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- AM05260PU-L - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AM05260PU-L, RRID:AB_11145941
- Product name
- anti LEF1
- Antibody type
- Monoclonal
- Antigen
- Full length recombinant LEF-1 protein.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- REMB1
- Vial size
- 0.25 mg
- Concentration
- Lot specific
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis using LEF-1 Antibody Cat.-No AM05260PU-L (Clone REMB1) on Jurkat cell lysate (AS00010LY-N) at 5 µg/ml.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunofluorescence staining of Cos-1 cells transfected with (top to bottom) full length hLEF-1, a LEF-1 mutant missing the nuclear localization signal, TCF-4 and TCF-1. Cells were stained with LEF-1 Antibody Cat.-No AM05260PU-L (Clone REMB1) and visualized with FITC conjugated mouse IgG. DAPI was used to detect all nuclei. The LEF-1 clone REMB1 recognized an epitope in the beta catenin binding domain that is present only in LEF-1.
