698701

antibody from BioLegend

Targeting: TCF12 bHLHb20, HEB, HsT17266, HTF4, p64

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

698701

Provider

BioLegend

Proper citation

BioLegend Cat#698701, RRID:AB_2687319

Product name

Purified anti-TCF12

Antibody type

Monoclonal

Reactivity

Human

Host

Rat

Conjugate

Unconjugated

Antibody clone number

W16168A

Vial size

25 µg

Concentration

0.5 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Western blot

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Total cell lysate (15µg protein) from MCF-7 (negative control), Jurkat and EL4 cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:500 diluted (1µg/mL) purified anti-TCF12 antibody (clone W16168A) or competitor’s antibody used at manufactures recommended concentration (upper). Proteins were visualized by chemiluminescence detection using 1:3000 diluted HRP goat anti-rat-IgG secondary antibody for clone W16168A or a 1:3000 diluted donkey anti-rabbit IgG Antibody conjugated to HRP for competitor’s antibody (upper). Direct-Blot™ HRP anti-β-actin antibody (1:2000 diluted, clone 2F1-1) was used as a loading control (lower). Lane M: Molecular weight ladder.

Conjugate

Unconjugated