- PA5-21039 - Invitrogen Antibodies TCF12 antibody

Submitted references Nuclear Wiskott-Aldrich syndrome protein co-regulates T cell factor 1-mediated transcription in T cells.

Kuznetsov NV, Almuzzaini B, Kritikou JS, Baptista MAP, Oliveira MMS, Keszei M, Snapper SB, Percipalle P, Westerberg LS
Genome medicine 2017 Oct 27;9(1):91

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of HeLa cell lysate using a TCF12 polyclonal antibody (Product # PA5-21039) at (A) 0.5 and (B) 1 µg/mL.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of TCF12 in HeLa cell lysate with TCF12 Polyclonal Antibody (Product # PA5-21039) at (A) 0.5 and (B) 1 µg/mL.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry of TCF12 in HeLa cells with TCF12 Polyclonal Antibody (Product # PA5-21039) at 10 µg/mL.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 2 Transcription matrix for selected WASp enriched genes. a Bioinformatic association of WASp ChIP-seq peaks from our datasets with activating (A) and/or repressive (R) epigenetic regulatory marks, RNA Polymerase II and TFs peaks and lamin B1 marks in thymocytes (T) and spleen CD4 + cells (S). b Association of WASp ChIP-seq peaks in thymocytes of Tcf12 , Vav2 , and Wasf2 with RNA Polymerase II ChIP-Seq signal by TFBS ENCODE/LICR resource at UCSC genome browser

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 6 The activation status of nuclear WASp affects TCF1-mediated Tcf12 expression. a TCF12 in NE and CE from WT, WASp KO, WASp I296T , and WASp L272P thymocytes. These data were repeated twice with independent biological replicates. Detection of GAPDH was used as a sample loading control for total lysate and for cytosolic extract. Detection of histone H3 was used as a sample loading control for total lysate and for nuclear extract. The quantification of nuclear and cytosolic TCF12 in the western blot is shown in the right panel . b Consensus DNA motif determined by WASp ChIP-seq data analysis and the canonical TCF1 DNA-binding site. c Proximity of WASp ChIP-seq gene peak positions and TCF1-binding sites in Tcf12 intron 3. d TCF1 in NE and CE from WT, WASp KO, WASp I296T , and WASp L272P thymocytes. These data were repeated twice with independent biological replicates. Detection of GAPDH was used as a sample loading control for total lysate and for cytosolic extract. Detection of histone H3 was used as a sample loading control for total lysate and for nuclear extract. The quantification of nuclear and cytosolic TCF1 in the western blot is shown in the right panel. e Co-IP of WASp and TCF1 from thymocyte nuclear extracts. These data were repeated three times with independent biological replicates. Purified mouse IgG 2A was used as the isotype Ab-negative control IP. Heavy chain of IgG (IgGhc) is observed in eluted IP samples. Additional file 1 : Figure S6 and S7 and Additiona

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 3 Variation of distances from WASp ChIP-Seq gene peaks to nearest exon(s). Selected examples of genes with ( a ) shorter distance range (0.5-5 kb) and ( b ) longer distance range (5-50 kb) between WASp ChIP-seq gene peak position and nearest exon. Additional file 1 : Figure S4

PA5-21039

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