AF3075

antibody from R&D Systems

Targeting: SOX9 CMD1, CMPD1, SRA1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF3075

Provider

R&D Systems

Product name

Human SOX9 Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects human SOX9 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human (rh) SOX10 is observed, and less than 15% rhSOX11, rhSOX12, and rhSOX15 is observed, and less than 5% cross-reactivity with rhSOX1, rhSOX2, rhSOX3, rhSOX5, rhSOX6, rhSOX7, rhSOX8, rhSOX13, rhSOX17, rhSOX18, rhSOX21, and rhSOX30 is observed.

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Antigen sequence

P48436


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Isolation and propagation of primary human cholangiocyte organoids for the generation of bioengineered biliary tissue.

Tysoe OC, Justin AW, Brevini T, Chen SE, Mahbubani KT, Frank AK, Zedira H, Melum E, Saeb-Parsy K, Markaki AE, Vallier L, Sampaziotis F
Nature protocols 2019 Jun;14(6):1884-1925

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Endothelial proteolytic activity and interaction with non-resorbing osteoclasts mediate bone elongation.

Romeo SG, Alawi KM, Rodrigues J, Singh A, Kusumbe AP, Ramasamy SK
Nature cell biology 2019 Apr;21(4):430-441

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3D Modeling of Esophageal Development using Human PSC-Derived Basal Progenitors Reveals a Critical Role for Notch Signaling.

Zhang Y, Yang Y, Jiang M, Huang SX, Zhang W, Al Alam D, Danopoulos S, Mori M, Chen YW, Balasubramanian R, Chuva de Sousa Lopes SM, Serra C, Bialecka M, Kim E, Lin S, Toste de Carvalho ALR, Riccio PN, Cardoso WV, Zhang X, Snoeck HW, Que J
Cell stem cell 2018 Oct 4;23(4):516-529.e5

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Mutations in GFAP Disrupt the Distribution and Function of Organelles in Human Astrocytes.

Jones JR, Kong L, Hanna MG 4th, Hoffman B, Krencik R, Bradley R, Hagemann T, Choi J, Doers M, Dubovis M, Sherafat MA, Bhattacharyya A, Kendziorski C, Audhya A, Messing A, Zhang SC
Cell reports 2018 Oct 23;25(4):947-958.e4

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Physical positioning markedly enhances brain transduction after intrathecal AAV9 infusion.

Castle MJ, Cheng Y, Asokan A, Tuszynski MH
Science advances 2018 Nov;4(11):eaau9859

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Restorative effects of human neural stem cell grafts on the primate spinal cord.

Rosenzweig ES, Brock JH, Lu P, Kumamaru H, Salegio EA, Kadoya K, Weber JL, Liang JJ, Moseanko R, Hawbecker S, Huie JR, Havton LA, Nout-Lomas YS, Ferguson AR, Beattie MS, Bresnahan JC, Tuszynski MH
Nature medicine 2018 May;24(4):484-490

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Developmental History Provides a Roadmap for the Emergence of Tumor Plasticity.

Tata PR, Chow RD, Saladi SV, Tata A, Konkimalla A, Bara A, Montoro D, Hariri LP, Shih AR, Mino-Kenudson M, Mou H, Kimura S, Ellisen LW, Rajagopal J
Developmental cell 2018 Mar 26;44(6):679-693.e5

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Highly efficient methods to obtain homogeneous dorsal neural progenitor cells from human and mouse embryonic stem cells and induced pluripotent stem cells.

Zhang M, Ngo J, Pirozzi F, Sun YP, Wynshaw-Boris A
Stem cell research & therapy 2018 Mar 15;9(1):67

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In utero electroporation induces cell death and alters embryonic microglia morphology and expression signatures in the developing hypothalamus.

Rosin JM, Kurrasch DM
Journal of neuroinflammation 2018 Jun 12;15(1):181

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Meis1 Coordinates Cerebellar Granule Cell Development by Regulating Pax6 Transcription, BMP Signaling and Atoh1 Degradation.

Owa T, Taya S, Miyashita S, Yamashita M, Adachi T, Yamada K, Yokoyama M, Aida S, Nishioka T, Inoue YU, Goitsuka R, Nakamura T, Inoue T, Kaibuchi K, Hoshino M
The Journal of neuroscience : the official journal of the Society for Neuroscience 2018 Jan 31;38(5):1277-1294

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Enhanced differentiation of human pluripotent stem cells into pancreatic progenitors co-expressing PDX1 and NKX6.1.

Memon B, Karam M, Al-Khawaga S, Abdelalim EM
Stem cell research & therapy 2018 Jan 23;9(1):15

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Complex bile duct network formation within liver decellularized extracellular matrix hydrogels.

Lewis PL, Su J, Yan M, Meng F, Glaser SS, Alpini GD, Green RM, Sosa-Pineda B, Shah RN
Scientific reports 2018 Aug 15;8(1):12220

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Differentiation of human pluripotent stem cells into two distinct NKX6.1 populations of pancreatic progenitors.

Aigha II, Memon B, Elsayed AK, Abdelalim EM
Stem cell research & therapy 2018 Apr 3;9(1):83

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An Integrative Developmental Genomics and Systems Biology Approach to Identify an In Vivo Sox Trio-Mediated Gene Regulatory Network in Murine Embryos.

Lee WJ, Chatterjee S, Yap SP, Lim SL, Xing X, Kraus P, Sun W, Hu X, Sivakamasundari V, Chan HY, Kolatkar PR, Prabhakar S, Lufkin T
BioMed research international 2017;2017:8932583

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YAP is essential for mechanical force production and epithelial cell proliferation during lung branching morphogenesis.

Lin C, Yao E, Zhang K, Jiang X, Croll S, Thompson-Peer K, Chuang PT
eLife 2017 Mar 21;6

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Fate mapping neurons and glia derived from Dbx1-expressing progenitors in mouse preBötzinger complex.

Kottick A, Martin CA, Del Negro CA
Physiological reports 2017 Jun;5(11)

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SOX9 Is an Astrocyte-Specific Nuclear Marker in the Adult Brain Outside the Neurogenic Regions.

Sun W, Cornwell A, Li J, Peng S, Osorio MJ, Aalling N, Wang S, Benraiss A, Lou N, Goldman SA, Nedergaard M
The Journal of neuroscience : the official journal of the Society for Neuroscience 2017 Apr 26;37(17):4493-4507

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Lung epithelial tip progenitors integrate glucocorticoid- and STAT3-mediated signals to control progeny fate.

Laresgoiti U, Nikolić MZ, Rao C, Brady JL, Richardson RV, Batchen EJ, Chapman KE, Rawlins EL
Development (Cambridge, England) 2016 Oct 15;143(20):3686-3699

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The development and plasticity of alveolar type 1 cells.

Yang J, Hernandez BJ, Martinez Alanis D, Narvaez del Pilar O, Vila-Ellis L, Akiyama H, Evans SE, Ostrin EJ, Chen J
Development (Cambridge, England) 2016 Jan 1;143(1):54-65

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Expression of SOX9 and CDX2 in nongoblet columnar-lined esophagus predicts the detection of Barrett's esophagus during follow-up.

Zhang X, Westerhoff M, Hart J
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 2015 May;28(5):654-61

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Acellular lung scaffolds direct differentiation of endoderm to functional airway epithelial cells: requirement of matrix-bound HS proteoglycans.

Shojaie S, Ermini L, Ackerley C, Wang J, Chin S, Yeganeh B, Bilodeau M, Sambi M, Rogers I, Rossant J, Bear CE, Post M
Stem cell reports 2015 Mar 10;4(3):419-30

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Two nested developmental waves demarcate a compartment boundary in the mouse lung.

Alanis DM, Chang DR, Akiyama H, Krasnow MA, Chen J
Nature communications 2014 May 29;5:3923

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Endothelial Notch activity promotes angiogenesis and osteogenesis in bone.

Ramasamy SK, Kusumbe AP, Wang L, Adams RH
Nature 2014 Mar 20;507(7492):376-380

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The hippo pathway effector Yap controls patterning and differentiation of airway epithelial progenitors.

Mahoney JE, Mori M, Szymaniak AD, Varelas X, Cardoso WV
Developmental cell 2014 Jul 28;30(2):137-50

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In vivo genome-wide analysis of multiple tissues identifies gene regulatory networks, novel functions and downstream regulatory genes for Bapx1 and its co-regulation with Sox9 in the mammalian vertebral column.

Chatterjee S, Sivakamasundari V, Yap SP, Kraus P, Kumar V, Xing X, Lim SL, Sng J, Prabhakar S, Lufkin T
BMC genomics 2014 Dec 5;15(1):1072

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Identification of SOX3 as an XX male sex reversal gene in mice and humans.

Sutton E, Hughes J, White S, Sekido R, Tan J, Arboleda V, Rogers N, Knower K, Rowley L, Eyre H, Rizzoti K, McAninch D, Goncalves J, Slee J, Turbitt E, Bruno D, Bengtsson H, Harley V, Vilain E, Sinclair A, Lovell-Badge R, Thomas P
The Journal of clinical investigation 2011 Jan;121(1):328-41

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The vanishing testis: a histomorphologic and clinical assessment.

Antic T, Hyjek EM, Taxy JB
American journal of clinical pathology 2011 Dec;136(6):872-80

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Chondrocytes from patients with osteoarthritis express typical extracellular matrix molecules once grown onto a three-dimensional hyaluronan-based scaffold.

Cavallo C, Desando G, Facchini A, Grigolo B
Journal of biomedical materials research. Part A 2010 Apr;93(1):86-95

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Cyclic GMP-dependent protein kinase II inhibits cell proliferation, Sox9 expression and Akt phosphorylation in human glioma cell lines.

Swartling FJ, Ferletta M, Kastemar M, Weiss WA, Westermark B
Oncogene 2009 Sep 3;28(35):3121-31

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A novel approach for the derivation of putative primordial germ cells and sertoli cells from human embryonic stem cells.

Bucay N, Yebra M, Cirulli V, Afrikanova I, Kaido T, Hayek A, Montgomery AM
Stem cells (Dayton, Ohio) 2009 Jan;27(1):68-77

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FOXL2 and SOX9 as parameters of female and male gonadal differentiation in patients with various forms of disorders of sex development (DSD).

Hersmus R, Kalfa N, de Leeuw B, Stoop H, Oosterhuis JW, de Krijger R, Wolffenbuttel KP, Drop SL, Veitia RA, Fellous M, Jaubert F, Looijenga LH
The Journal of pathology 2008 May;215(1):31-8

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human SOX9 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, KATO-III human gastric carcinoma cell line, COLO 205 human colorectal adenocarcinoma cell line, and Hep3B human hepatocellular carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human SOX9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3075) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for SOX9 at approximately 75 kDa (as indicated). GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human SOX9 by Simple Western^TM. Simple Western lane view shows lysates of normal adjacent tissue and Crohns tissue, loaded at 0.2 mg/mL. A specific band was detected for SOX9 at approximately 107 kDa (as indicated) using 20 µg/mL of Goat Anti-Human SOX9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3075) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Immunocytochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

SOX9 in HEK293 Human Cell Line. SOX9 was detected in immersion fixed HEK293 human embryonic kidney cell line using 10 µg/mL Human SOX9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3075) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

SOX9 in BG01V Human Embryonic Stem Cells. SOX9 was detected in immersion fixed BG01V human embryonic stem cells differentiated into early proximal lung progenitor cells using Goat Anti-Human SOX9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3075) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.