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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
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- Product number
- H00000405-B01P - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00000405-B01P, RRID:AB_1672843
- Product name
- ARNT purified MaxPab mouse polyclonal antibody (B01P)
- Antibody type
- Polyclonal
- Description
- Mouse polyclonal antibody raised against a full-length human ARNT protein.
- Antigen sequence
MAATTANPEMTSDVPSLGPAIASGNSGPGIQGGGA
IVQRAIKRRPGLDFDDDGEGNSKFLRCDDDQMSND
KERFARSDDEQSSADKERLARENHSEIERRRRNKM
TAYITELSDMVPTCSALARKPDKLTILRMAVSHMK
SLRGTGNTSTDGSYKPSFLTDQELKHLILEAADGF
LFIVSCETGRVVYVSDSVTPVLNQPQSEWFGSTLY
DQVHPDDVDKLREQLSTSENALTGRILDLKTGTVK
KEGQQSSMRMCMGSRRSFICRMRCGSSSVDPVSVN
RLSFVRNRCRNGLGSVKDGEPHFVVVHCTGYIKAW
PPAGVSLPDDDPEAGQGSKFCLVAIGRLQVTSSPN
CTDMSNVCQPTEFISRHNIEGIFTFVDHRCVATVG
YQPQELLGKNIVEFCHPEDQQLLRDSFQQVVKLKG
QVLSVMFRFRSKNQEWLWMRTSSFTFQNPYSDEIE
YIICTNTNVKNSSQEPRPTLSNTIQRPQLGPTANL
PLEMGSGQLAPRQQQQQTELDMVPGRDGLASYNHS
QVVQPVTTTGPEHSKPLEKSDGLFAQDRDPRFSEI
YHNINADQSKGISSSTVPATQQLFSQGNTFPPTPR
PAENFRNSGLAPPVTIVQPSASAGQMLAQISRHSN
PTQGATPTWTPTTRSGFSAQQVATQATAKTRTSQF
GVGSFQTPSSFSSMSLPGAPTASPGAAAYPSLTNR
GSNFAPETGQTAGQFQTRTAEGVGVWPQWQGQQPH
HRSSSSEQHVQQPPAQQPGQPEVFQEMLSMLGDQS
NSYNNEEFPDLTMFPPFSE- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references A cyclic peptide inhibitor of HIF-1 heterodimerization that inhibits hypoxia signaling in cancer cells.
Miranda E, Nordgren IK, Male AL, Lawrence CE, Hoakwie F, Cuda F, Court W, Fox KR, Townsend PA, Packham GK, Eccles SA, Tavassoli A
Journal of the American Chemical Society 2013 Jul 17;135(28):10418-25
Journal of the American Chemical Society 2013 Jul 17;135(28):10418-25
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of ARNT expression in transfected 293T cell line (H00000405-T01) by ARNT MaxPab polyclonal antibody.Lane 1: ARNT transfected lysate(86.79 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- ARNT MaxPab polyclonal antibody. Western Blot analysis of ARNT expression in HepG2.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- ARNT MaxPab polyclonal antibody. Western Blot analysis of ARNT expression in HeLa.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of purified MaxPab antibody to ARNT on HeLa cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
