M02263

antibody from Boster Biological Technology

Targeting: ARNT bHLHe2, HIF-1beta

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

M02263

Provider

Boster Biological Technology

Product name

Anti-HIF-1 beta ARNT Rabbit Monoclonal Antibody

Antibody type

Monoclonal

Description

Monoclonal antibody for HIF 1 BETA/ARNT detection. Host: Rabbit.Size: 100ug/vial. Tested applications: IHC, WB. Reactive species: Human HIF 1 BETA/ARNT information: Molecular Weight: 86636 MW; Subcellular Localization: Nucleus.

Reactivity

Human

Host

Rabbit

Antibody clone number

EAD-1

Vial size

100ug/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. Avoid repeated freezing and thawing.

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human colon, using HIF-1 beta Antibody (M02263)ARNT was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ARNT Antibody (M02263)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Additional image