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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [38]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Chromatin Immunoprecipitation [1]
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- Product number
- AF2418 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat Olig2 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Olig2 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) Olig3 is observed.
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q13516- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references BMP6 expression in the adult rat central nervous system.
Oligodendrocyte gene expression is reduced by and influences effects of chronic social stress in mice.
Microglia are an essential component of the neuroprotective scar that forms after spinal cord injury.
Human ESC-Derived Chimeric Mouse Models of Huntington's Disease Reveal Cell-Intrinsic Defects in Glial Progenitor Cell Differentiation.
Treatment of Human Glioblastoma with a Live Attenuated Zika Virus Vaccine Candidate.
NF-κB p65 directs sex-specific neuroprotection in human neurons.
A TLR/AKT/FoxO3 immune tolerance-like pathway disrupts the repair capacity of oligodendrocyte progenitors.
Histone Deacetylases 1 and 2 Regulate Microglia Function during Development, Homeostasis, and Neurodegeneration in a Context-Dependent Manner.
Myostatin expression in the adult rat central nervous system.
Disease-specific oligodendrocyte lineage cells arise in multiple sclerosis.
Targeting Glioma Stem Cell-Derived Pericytes Disrupts the Blood-Tumor Barrier and Improves Chemotherapeutic Efficacy.
The doublesex-related Dmrta2 safeguards neural progenitor maintenance involving transcriptional regulation of Hes1.
The intellectual disability protein PAK3 regulates oligodendrocyte precursor cell differentiation.
Delayed treatment of secondary degeneration following acute optic nerve transection using a combination of ion channel inhibitors.
Treatment Outcome and Prognostic Molecular Markers of Supratentorial Primitive Neuroectodermal Tumors.
Olig2 is expressed late in human eosinophil development and controls Siglec-8 expression.
RBPJ maintains brain tumor-initiating cells through CDK9-mediated transcriptional elongation.
Brain tissue banking for stem cells for our future.
Patched1 and Patched2 inhibit Smoothened non-cell autonomously.
A cord blood monocyte-derived cell therapy product accelerates brain remyelination.
Connective-Tissue Growth Factor (CTGF/CCN2) Induces Astrogenesis and Fibronectin Expression of Embryonic Neural Cells In Vitro.
A mouse model of L-2-hydroxyglutaric aciduria, a disorder of metabolite repair.
Notch signaling regulates motor neuron differentiation of human embryonic stem cells.
Mitochondrial control by DRP1 in brain tumor initiating cells.
Temporal identity transition from Purkinje cell progenitors to GABAergic interneuron progenitors in the cerebellum.
Nitric oxide-induced neuronal to glial lineage fate-change depends on NRSF/REST function in neural progenitor cells.
Glioma cancer stem cells secrete Gremlin1 to promote their maintenance within the tumor hierarchy.
BMP signaling induces astrocytic differentiation of clinically derived oligodendroglioma propagating cells.
Phage display discovery of novel molecular targets in glioblastoma-initiating cells.
Safety of epicenter versus intact parenchyma as a transplantation site for human neural stem cells for spinal cord injury therapy.
The reactivity, distribution and abundance of Non-astrocytic Inner Retinal Glial (NIRG) cells are regulated by microglia, acute damage, and IGF1.
N-Myc and GCN5 regulate significantly overlapping transcriptional programs in neural stem cells.
Protein tyrosine phosphatase receptor type z negatively regulates oligodendrocyte differentiation and myelination.
Human neural stem cells differentiate and promote locomotor recovery in an early chronic spinal cord injury NOD-scid mouse model.
A multicolor panel of novel lentiviral "gene ontology" (LeGO) vectors for functional gene analysis.
Increased neurogenesis and astrogenesis from neural progenitor cells grafted in the hippocampus of GFAP-/- Vim-/- mice.
Characterization of progenitor domains in the developing mouse thalamus.
Leukemia inhibitory factor promotes neural stem cell self-renewal in the adult brain.
Hayashi Y, Mikawa S, Ogawa C, Masumoto K, Katou F, Sato K
Journal of chemical neuroanatomy 2019 Jul;98:41-54
Journal of chemical neuroanatomy 2019 Jul;98:41-54
Oligodendrocyte gene expression is reduced by and influences effects of chronic social stress in mice.
Cathomas F, Azzinnari D, Bergamini G, Sigrist H, Buerge M, Hoop V, Wicki B, Goetze L, Soares S, Kukelova D, Seifritz E, Goebbels S, Nave KA, Ghandour MS, Seoighe C, Hildebrandt T, Leparc G, Klein H, Stupka E, Hengerer B, Pryce CR
Genes, brain, and behavior 2019 Jan;18(1):e12475
Genes, brain, and behavior 2019 Jan;18(1):e12475
Microglia are an essential component of the neuroprotective scar that forms after spinal cord injury.
Bellver-Landete V, Bretheau F, Mailhot B, Vallières N, Lessard M, Janelle ME, Vernoux N, Tremblay MÈ, Fuehrmann T, Shoichet MS, Lacroix S
Nature communications 2019 Jan 31;10(1):518
Nature communications 2019 Jan 31;10(1):518
Human ESC-Derived Chimeric Mouse Models of Huntington's Disease Reveal Cell-Intrinsic Defects in Glial Progenitor Cell Differentiation.
Osipovitch M, Asenjo Martinez A, Mariani JN, Cornwell A, Dhaliwal S, Zou L, Chandler-Militello D, Wang S, Li X, Benraiss SJ, Agate R, Lampp A, Benraiss A, Windrem MS, Goldman SA
Cell stem cell 2019 Jan 3;24(1):107-122.e7
Cell stem cell 2019 Jan 3;24(1):107-122.e7
Treatment of Human Glioblastoma with a Live Attenuated Zika Virus Vaccine Candidate.
Chen Q, Wu J, Ye Q, Ma F, Zhu Q, Wu Y, Shan C, Xie X, Li D, Zhan X, Li C, Li XF, Qin X, Zhao T, Wu H, Shi PY, Man J, Qin CF
mBio 2018 Sep 18;9(5)
mBio 2018 Sep 18;9(5)
NF-κB p65 directs sex-specific neuroprotection in human neurons.
Ruiz-Perera LM, Schneider L, Windmöller BA, Müller J, Greiner JFW, Kaltschmidt C, Kaltschmidt B
Scientific reports 2018 Oct 30;8(1):16012
Scientific reports 2018 Oct 30;8(1):16012
A TLR/AKT/FoxO3 immune tolerance-like pathway disrupts the repair capacity of oligodendrocyte progenitors.
Srivastava T, Diba P, Dean JM, Banine F, Shaver D, Hagen M, Gong X, Su W, Emery B, Marks DL, Harris EN, Baggenstoss B, Weigel PH, Sherman LS, Back SA
The Journal of clinical investigation 2018 May 1;128(5):2025-2041
The Journal of clinical investigation 2018 May 1;128(5):2025-2041
Histone Deacetylases 1 and 2 Regulate Microglia Function during Development, Homeostasis, and Neurodegeneration in a Context-Dependent Manner.
Datta M, Staszewski O, Raschi E, Frosch M, Hagemeyer N, Tay TL, Blank T, Kreutzfeldt M, Merkler D, Ziegler-Waldkirch S, Matthias P, Meyer-Luehmann M, Prinz M
Immunity 2018 Mar 20;48(3):514-529.e6
Immunity 2018 Mar 20;48(3):514-529.e6
Myostatin expression in the adult rat central nervous system.
Hayashi Y, Mikawa S, Ogawa C, Masumoto K, Katou F, Sato K
Journal of chemical neuroanatomy 2018 Dec;94:125-138
Journal of chemical neuroanatomy 2018 Dec;94:125-138
Disease-specific oligodendrocyte lineage cells arise in multiple sclerosis.
Falcão AM, van Bruggen D, Marques S, Meijer M, Jäkel S, Agirre E, Samudyata, Floriddia EM, Vanichkina DP, Ffrench-Constant C, Williams A, Guerreiro-Cacais AO, Castelo-Branco G
Nature medicine 2018 Dec;24(12):1837-1844
Nature medicine 2018 Dec;24(12):1837-1844
Targeting Glioma Stem Cell-Derived Pericytes Disrupts the Blood-Tumor Barrier and Improves Chemotherapeutic Efficacy.
Zhou W, Chen C, Shi Y, Wu Q, Gimple RC, Fang X, Huang Z, Zhai K, Ke SQ, Ping YF, Feng H, Rich JN, Yu JS, Bao S, Bian XW
Cell stem cell 2017 Nov 2;21(5):591-603.e4
Cell stem cell 2017 Nov 2;21(5):591-603.e4
The doublesex-related Dmrta2 safeguards neural progenitor maintenance involving transcriptional regulation of Hes1.
Young FI, Keruzore M, Nan X, Gennet N, Bellefroid EJ, Li M
Proceedings of the National Academy of Sciences of the United States of America 2017 Jul 11;114(28):E5599-E5607
Proceedings of the National Academy of Sciences of the United States of America 2017 Jul 11;114(28):E5599-E5607
The intellectual disability protein PAK3 regulates oligodendrocyte precursor cell differentiation.
Maglorius Renkilaraj MRL, Baudouin L, Wells CM, Doulazmi M, Wehrlé R, Cannaya V, Bachelin C, Barnier JV, Jia Z, Nait Oumesmar B, Dusart I, Bouslama-Oueghlani L
Neurobiology of disease 2017 Feb;98:137-148
Neurobiology of disease 2017 Feb;98:137-148
Delayed treatment of secondary degeneration following acute optic nerve transection using a combination of ion channel inhibitors.
Yates NJ, Giacci MK, O'Hare Doig RL, Chiha W, Ashworth BE, Kenna J, Bartlett CA, Fitzgerald M
Neural regeneration research 2017 Feb;12(2):307-316
Neural regeneration research 2017 Feb;12(2):307-316
Treatment Outcome and Prognostic Molecular Markers of Supratentorial Primitive Neuroectodermal Tumors.
Choi SH, Kim SH, Shim KW, Han JW, Choi J, Kim DS, Lyu CJ, Kim JW, Suh CO, Cho J
PloS one 2016;11(4):e0153443
PloS one 2016;11(4):e0153443
Olig2 is expressed late in human eosinophil development and controls Siglec-8 expression.
Hwang SM, Uhm TG, Lee SK, Kong SK, Jung KH, Binas B, Chai YG, Park SW, Chung IY
Journal of leukocyte biology 2016 Oct;100(4):711-723
Journal of leukocyte biology 2016 Oct;100(4):711-723
RBPJ maintains brain tumor-initiating cells through CDK9-mediated transcriptional elongation.
Xie Q, Wu Q, Kim L, Miller TE, Liau BB, Mack SC, Yang K, Factor DC, Fang X, Huang Z, Zhou W, Alazem K, Wang X, Bernstein BE, Bao S, Rich JN
The Journal of clinical investigation 2016 Jul 1;126(7):2757-72
The Journal of clinical investigation 2016 Jul 1;126(7):2757-72
Brain tissue banking for stem cells for our future.
Palmero E, Palmero S, Murrell W
Scientific reports 2016 Dec 19;6:39394
Scientific reports 2016 Dec 19;6:39394
Patched1 and Patched2 inhibit Smoothened non-cell autonomously.
Roberts B, Casillas C, Alfaro AC, Jägers C, Roelink H
eLife 2016 Aug 23;5
eLife 2016 Aug 23;5
A cord blood monocyte-derived cell therapy product accelerates brain remyelination.
Saha A, Buntz S, Scotland P, Xu L, Noeldner P, Patel S, Wollish A, Gunaratne A, Gentry T, Troy J, Matsushima GK, Kurtzberg J, Balber AE
JCI insight 2016 Aug 18;1(13):e86667
JCI insight 2016 Aug 18;1(13):e86667
Connective-Tissue Growth Factor (CTGF/CCN2) Induces Astrogenesis and Fibronectin Expression of Embryonic Neural Cells In Vitro.
Mendes FA, Coelho Aguiar JM, Kahn SA, Reis AH, Dubois LG, Romão LF, Ferreira LS, Chneiweiss H, Moura Neto V, Abreu JG
PloS one 2015;10(8):e0133689
PloS one 2015;10(8):e0133689
A mouse model of L-2-hydroxyglutaric aciduria, a disorder of metabolite repair.
Rzem R, Achouri Y, Marbaix E, Schakman O, Wiame E, Marie S, Gailly P, Vincent MF, Veiga-da-Cunha M, Van Schaftingen E
PloS one 2015;10(3):e0119540
PloS one 2015;10(3):e0119540
Notch signaling regulates motor neuron differentiation of human embryonic stem cells.
Ben-Shushan E, Feldman E, Reubinoff BE
Stem cells (Dayton, Ohio) 2015 Feb;33(2):403-15
Stem cells (Dayton, Ohio) 2015 Feb;33(2):403-15
Mitochondrial control by DRP1 in brain tumor initiating cells.
Xie Q, Wu Q, Horbinski CM, Flavahan WA, Yang K, Zhou W, Dombrowski SM, Huang Z, Fang X, Shi Y, Ferguson AN, Kashatus DF, Bao S, Rich JN
Nature neuroscience 2015 Apr;18(4):501-10
Nature neuroscience 2015 Apr;18(4):501-10
Temporal identity transition from Purkinje cell progenitors to GABAergic interneuron progenitors in the cerebellum.
Seto Y, Nakatani T, Masuyama N, Taya S, Kumai M, Minaki Y, Hamaguchi A, Inoue YU, Inoue T, Miyashita S, Fujiyama T, Yamada M, Chapman H, Campbell K, Magnuson MA, Wright CV, Kawaguchi Y, Ikenaka K, Takebayashi H, Ishiwata S, Ono Y, Hoshino M
Nature communications 2014;5:3337
Nature communications 2014;5:3337
Nitric oxide-induced neuronal to glial lineage fate-change depends on NRSF/REST function in neural progenitor cells.
Bergsland M, Covacu R, Perez Estrada C, Svensson M, Brundin L
Stem cells (Dayton, Ohio) 2014 Sep;32(9):2539-49
Stem cells (Dayton, Ohio) 2014 Sep;32(9):2539-49
Glioma cancer stem cells secrete Gremlin1 to promote their maintenance within the tumor hierarchy.
Yan K, Wu Q, Yan DH, Lee CH, Rahim N, Tritschler I, DeVecchio J, Kalady MF, Hjelmeland AB, Rich JN
Genes & development 2014 May 15;28(10):1085-100
Genes & development 2014 May 15;28(10):1085-100
BMP signaling induces astrocytic differentiation of clinically derived oligodendroglioma propagating cells.
Srikanth M, Kim J, Das S, Kessler JA
Molecular cancer research : MCR 2014 Feb;12(2):283-94
Molecular cancer research : MCR 2014 Feb;12(2):283-94
Phage display discovery of novel molecular targets in glioblastoma-initiating cells.
Liu JK, Lubelski D, Schonberg DL, Wu Q, Hale JS, Flavahan WA, Mulkearns-Hubert EE, Man J, Hjelmeland AB, Yu J, Lathia JD, Rich JN
Cell death and differentiation 2014 Aug;21(8):1325-39
Cell death and differentiation 2014 Aug;21(8):1325-39
Safety of epicenter versus intact parenchyma as a transplantation site for human neural stem cells for spinal cord injury therapy.
Piltti KM, Salazar DL, Uchida N, Cummings BJ, Anderson AJ
Stem cells translational medicine 2013 Mar;2(3):204-16
Stem cells translational medicine 2013 Mar;2(3):204-16
The reactivity, distribution and abundance of Non-astrocytic Inner Retinal Glial (NIRG) cells are regulated by microglia, acute damage, and IGF1.
Zelinka CP, Scott MA, Volkov L, Fischer AJ
PloS one 2012;7(9):e44477
PloS one 2012;7(9):e44477
N-Myc and GCN5 regulate significantly overlapping transcriptional programs in neural stem cells.
Martínez-Cerdeño V, Lemen JM, Chan V, Wey A, Lin W, Dent SR, Knoepfler PS
PloS one 2012;7(6):e39456
PloS one 2012;7(6):e39456
Protein tyrosine phosphatase receptor type z negatively regulates oligodendrocyte differentiation and myelination.
Kuboyama K, Fujikawa A, Masumura M, Suzuki R, Matsumoto M, Noda M
PloS one 2012;7(11):e48797
PloS one 2012;7(11):e48797
Human neural stem cells differentiate and promote locomotor recovery in an early chronic spinal cord injury NOD-scid mouse model.
Salazar DL, Uchida N, Hamers FP, Cummings BJ, Anderson AJ
PloS one 2010 Aug 18;5(8):e12272
PloS one 2010 Aug 18;5(8):e12272
A multicolor panel of novel lentiviral "gene ontology" (LeGO) vectors for functional gene analysis.
Weber K, Bartsch U, Stocking C, Fehse B
Molecular therapy : the journal of the American Society of Gene Therapy 2008 Apr;16(4):698-706
Molecular therapy : the journal of the American Society of Gene Therapy 2008 Apr;16(4):698-706
Increased neurogenesis and astrogenesis from neural progenitor cells grafted in the hippocampus of GFAP-/- Vim-/- mice.
Widestrand A, Faijerson J, Wilhelmsson U, Smith PL, Li L, Sihlbom C, Eriksson PS, Pekny M
Stem cells (Dayton, Ohio) 2007 Oct;25(10):2619-27
Stem cells (Dayton, Ohio) 2007 Oct;25(10):2619-27
Characterization of progenitor domains in the developing mouse thalamus.
Vue TY, Aaker J, Taniguchi A, Kazemzadeh C, Skidmore JM, Martin DM, Martin JF, Treier M, Nakagawa Y
The Journal of comparative neurology 2007 Nov 1;505(1):73-91
The Journal of comparative neurology 2007 Nov 1;505(1):73-91
Leukemia inhibitory factor promotes neural stem cell self-renewal in the adult brain.
Bauer S, Patterson PH
The Journal of neuroscience : the official journal of the Society for Neuroscience 2006 Nov 15;26(46):12089-99
The Journal of neuroscience : the official journal of the Society for Neuroscience 2006 Nov 15;26(46):12089-99
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Olig2 and Oligodendrocyte Marker O4 in Rat Cortical Stem Cells. Olig2 and Oligodendrocyte Marker O4 were detected in 7 day differentiated rat cortical stem cells using 10 µg/mL Goat Anti-Human/Mouse/Rat Olig2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2418) and 10 µg/mL Goat Anti-Human/Mouse/Rat/Chicken O4 Monoclonal Antibody (Catalog # MAB1326). Cells were incubated with primary antibodies for 3 hours at room temperature. Cells were stained for Olig2 using the NorthernLights™ 637-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL002), and stained for O4 using an anti-mouse IgM secondary antibody (pseudo-stained green). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Olig2 in Mouse Embryo. Olig2 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Human/Mouse/Rat Olig2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2418) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to developing brain. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Olig2-regulated Genes by Chromatin Immunoprecipitation. A172 human glioblastoma cell line were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. Olig2/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human/Mouse/Rat Olig2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2418) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The p21 promoter was detected by standard PCR.
