NBP1-88890

antibody from Novus Biologicals

Targeting: ATP6V1B2 ATP6B2, HO57, VATB, Vma2, VPP3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-88890

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-88890, RRID:AB_11026573

Product name

Rabbit Polyclonal ATP6V1B2 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Specificity of human, mouse, rat ATP6V1B2 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: ATP6V1B2 Antibody [NBP1-88890] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: ATP6V1B2 Antibody [NBP1-88890] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: ATP6V1B2 Antibody [NBP1-88890] - Analysis in human cell lines SK-MEL-30 and MCF-7 using anti-ATP6V1B2 antibody. Corresponding ATP6V1B2 RNA-seq data are presented for the same cell lines. Loading control: anti-PFN1.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ATP6V1B2 Antibody [NBP1-88890] - Staining of human kidney shows strong cytoplasmic and membranous positivity in cells in tubules.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ATP6V1B2 Antibody [NBP1-88890] - Staining of human skeletal muscle shows low expression as expected.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ATP6V1B2 Antibody [NBP1-88890] - Staining in human cerebral cortex and skeletal muscle tissues using anti-ATP6V1B2 antibody. Corresponding ATP6V1B2 RNA-seq data are presented for the same tissues.