Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB29389 - Provider product page
- Provider
- Abnova Corporation
- Product name
- GABPA polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against recombinant human GABPA.
- Antigen sequence
EELIEIEIDGTEKAECTEESIVEQTYAPAECVSQA
IDINEPIGNLKKLLEPRLQCSLDAHEICLQDIQLD
PERSLFDQGVKTDGTVQLSVQVISYQGIEPKLNIL
EIVKPADTVEVVIDPDAHHAESEAHLVEEAQVITL
DGTK- Isotype
- IgG
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of Lane 1: Human cell line RT-4 Lane 2: Human cell line U-251MG sp Lane 3: Human liver tissue Lane 4: Human tonsil tissue with GABPA polyclonal antibody (Cat # PAB29389) at 1:100-1:250 dilution.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescent staining of human cell line U-251 MG with GABPA polyclonal antibody (Cat # PAB29389) at 1-4 ug/mL concentration shows positivity in nucleus but excluded from the nucleoli.
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human lymph node with GABPA polyclonal antibody (Cat # PAB29389) shows strong nuclear positivity in lymphoid cells outside reaction centra at 1:50-1:200 dilution.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)