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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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- Product number
- PA5-29826 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MiTF Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HeLa, mouse skin. Predicted reactivity: Mouse (92%), Rat (92%), Dog (95%), Pig (99%), Chicken (93%), Rhesus Monkey (100%), Bovine (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of MiTF was performed by separating 50 µg of Mouse tissue extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a MiTF Polyclonal Antibody (Product # PA5-29826) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using MiTF Polyclonal Antibody (Product # PA5-29826). Sample (30 µg of whole cell lysate). Lane A: HeLa. 10% SDS PAGE. MiTF Polyclonal Antibody (Product # PA5-29826) diluted at 1:2,000.
