PAB20198

antibody from Abnova Corporation

Targeting: ZNF146 OZF

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB20198

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB20198, RRID:AB_10962454

Product name

ZNF146 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant ZNF146.

Antigen sequence

LSQQRIYSGENPFACKVCGKVFSHKSNLTEHEHFH
TREKPFECNECGKAFSQKQYVIKHQNTHTGEKLFE
CNECGKSFSQKENLLTHQKIHTGEKPFECKDCGKA
FIQKSNLIR


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Liver, Lane 4: Tonsil with ZNF146 polyclonal antibody (Cat # PAB20198) at 1:250-1:500 dilution.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of cell lysates with ZNF146 polyclonal antibody (Cat # PAB20198) at 1:250-1:500 dilution.Lane 1 : NIH/3T3Lane 2 : NBT-II

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A-431 with ZNF146 polyclonal antibody (Cat # PAB20198) at 1-4 ug/mL dilution shows positivity in nucleoli.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human breast with ZNF146 polyclonal antibody (Cat # PAB20198) shows strong nuclear positivity in glandular cells at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)