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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- AP52812PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP52812PU-N, RRID:AB_11146832
- Product name
- anti NARS (N-term)
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 31-60 amino acids from the N-terminal region of human NARS
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.4 ml
- Concentration
- lot specific
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of NARS Antibody (N-term) Cat.-No AP52812PU-N in WiDr, HepG2 cell line lysates (35ug/lane).This demonstrates the NARS antibody detected the NARS protein (arrow).
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of NARS (arrow) using NARS Antibody (N-term) Cat.-No AP52812PU-N. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the NARS gene.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma reacted with NARS Antibody (N-term) Cat.-No AP52812PU-N, which was peroxidase conjugated to the secondary antibody and followed by DAB staining. This data demonstrates the use of NARS Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Flow cytometric analysis of WiDr cells using NARS Antibody (N-term) Cat.-No AP52812PU-N (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
