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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunoprecipitation [3]
- Immunohistochemistry [3]
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- Product number
- GTX110267 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX110267, RRID:AB_1950407
- Product name
- Glycine dehydrogenase antibody [N3C2-2], Internal
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Glycine dehydrogenase antibody detects Glycine dehydrogenase protein by western blot analysis.A. 30 ?g HeLa whole cell lysate/extractB. 30 ?g HepG2 whole cell lysate/extract 5 % SDS-PAGEGlycine dehydrogenase antibody (GTX110267) dilution: 1:500
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Whole cell extract (30 ?g) was separated by 5% SDS-PAGE, and the membrane was blotted with Glycine dehydrogenase antibody [N3C2-2], Internal (GTX110267) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Whole cell extract (30 ?g) was separated by 5% SDS-PAGE, and the membranes were blotted with Glycine dehydrogenase antibody [N3C2-2], Internal (GTX110267) diluted at 1:1000 and competitor's antibody (CST#12794) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Whole cell extract (30 ?g) was separated by 5% SDS-PAGE, and the membranes were blotted with Glycine dehydrogenase antibody [N3C2-2], Internal (GTX110267) diluted at 1:1000 and competitor's antibody (CST#12794) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Glycine dehydrogenase antibody [N3C2-2], Internal detects Glycine dehydrogenase protein at mitochondria by immunofluorescent analysis.Sample: Hep G2 cells were fixed in ice-cold MeOH for 5 min.Green: Glycine dehydrogenase protein stained by Glycine dehydrogenase antibody [N3C2-2], Internal (GTX110267) diluted at 1:400.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of Glycine dehydrogenase protein from HepG2 whole cell extracts using 5 ?g of Glycine dehydrogenase antibody [N3C2-2], Internal (GTX110267).Western blot analysis was performed using Glycine dehydrogenase antibody [N3C2-2], Internal (GTX110267).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
- Validation comment
- IP
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of Glycine dehydrogenase protein from HepG2 whole cell extracts using 5 £gg of Glycine dehydrogenase antibody [N3C2-2], Internal (GTX110267).Western blot analysis was performed using Glycine dehydrogenase antibody [N3C2-2], Internal (GTX110267).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of Glycine dehydrogenase protein from HepG2 whole cell extracts using 5 ?g of Glycine dehydrogenase antibody [N3C2-2], Internal (GTX110267).Western blot analysis was performed using Glycine dehydrogenase antibody [N3C2-2], Internal (GTX110267).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
- Validation comment
- IP
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Glycine dehydrogenase antibody [N3C2-2], Internal detects Glycine dehydrogenase protein at mitochondria in mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. Glycine dehydrogenase antibody [N3C2-2], Internal (GTX110267) diluted at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Glycine dehydrogenase antibody [N3C2-2], Internal detects Glycine dehydrogenase protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Glycine dehydrogenase antibody [N3C2-2], Internal (GTX110267) diluted at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Glycine dehydrogenase antibody [N3C2-2], Internal detects Glycine dehydrogenase protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Glycine dehydrogenase antibody [N3C2-2], Internal (GTX110267) diluted at 1:500.
