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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [26]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- MA5-11309 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- p57 Kip2 Monoclonal Antibody (57P06 (KP10))
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- MA5-11309 targets p57Kip2 in WB, IHC (P) and IP applications and shows reactivity with Human and mouse samples.
- Antibody clone number
- 57P06 (KP10)
- Concentration
- 0.2 mg/mL
Submitted references Epigenetic Dysregulation of Trophoblastic Gene Expression in Gestational Trophoblastic Disease.
Dysregulation of Placental Functions and Immune Pathways in Complete Hydatidiform Moles.
Ectopic Complete Hydatidiform Mole Presenting as an Adnexal Tumor in a Postmenopausal Patient.
Diagnostic utility of microsatellite genotyping for molar pregnancy testing.
The cyclin-dependent kinase inhibitor p57Kip2 regulates cell cycle exit, differentiation, and migration of embryonic cerebral cortical precursors.
Androgenetic complete mole with trisomy 13: report of a case with microsatellite genotyping and review of the literature.
MiR-221 and MiR-222 alterations in sporadic ovarian carcinoma: Relationship to CDKN1B, CDKNIC and overall survival.
Early molar pregnancy: experience in a large abortion service.
Comparison of fluorescence in situ hybridization, p57 immunostaining, flow cytometry, and digital image analysis for diagnosing molar and nonmolar products of conception.
The villous stromal constituents of complete hydatidiform mole differ histologically in very early pregnancy from the normally developing placenta.
Combination of immunohistochemistry and ploidy analysis to assist histopathological diagnosis of molar diseases.
SM22alpha-targeted deletion of bone morphogenetic protein receptor 1A in mice impairs cardiac and vascular development, and influences organogenesis.
Role of DNA content analysis and immunohistochemistry in the evaluation of the risk of unfavourable outcome in Wilms' tumours.
P57KIP2 immunostaining and molecular cytogenetics: combined approach aids in diagnosis of morphologically challenging cases with molar phenotype and in detecting androgenetic cell lines in mosaic/chimeric conceptions.
Cell cycle regulators show diagnostic and prognostic utility for differentiated thyroid cancer.
Glomerular sclerosis in kidneys with congenital nephrotic syndrome (NPHS1).
Multiplex short tandem repeat DNA analysis confirms the accuracy of p57(KIP2) immunostaining in the diagnosis of complete hydatidiform mole.
Diagnostic and pathogenetic significance of increased stromal apoptosis and incomplete vasculogenesis in complete hydatidiform moles in very early pregnancy periods.
E Proteins and Id2 converge on p57Kip2 to regulate cell cycle in neural cells.
Molecular and immunohistochemical analyses of the focal form of congenital hyperinsulinism.
Epigenetic down-regulation of CDKN1C/p57KIP2 in pancreatic ductal neoplasms identified by gene expression profiling.
p57KIP2 immunohistochemistry in early molar pregnancies: emphasis on its complementary role in the differential diagnosis of hydropic abortuses.
Downregulation of Bcl-2 by podocytes is associated with progressive glomerular injury and clinical indices of poor renal prognosis in human IgA nephropathy.
Downregulation of Bcl-2 by podocytes is associated with progressive glomerular injury and clinical indices of poor renal prognosis in human IgA nephropathy.
p57(KIP2) immunohistochemical staining of gestational trophoblastic tumours does not identify the type of the causative pregnancy.
p57kip2 is useful in the classification and differential diagnosis of complete and partial hydatidiform moles.
Szabolcsi Z, Demeter A, Kiraly P, Balogh A, Wilson ML, King JR, Hetey S, Gelencser Z, Matsuo K, Hargitai B, Mhawech-Fauceglia P, Hupuczi P, Szilagyi A, Papp Z, Roman LD, Cortessis VK, Than NG
Biomedicines 2021 Dec 17;9(12)
Biomedicines 2021 Dec 17;9(12)
Dysregulation of Placental Functions and Immune Pathways in Complete Hydatidiform Moles.
King JR, Wilson ML, Hetey S, Kiraly P, Matsuo K, Castaneda AV, Toth E, Krenacs T, Hupuczi P, Mhawech-Fauceglia P, Balogh A, Szilagyi A, Matko J, Papp Z, Roman LD, Cortessis VK, Than NG
International journal of molecular sciences 2019 Oct 10;20(20)
International journal of molecular sciences 2019 Oct 10;20(20)
Ectopic Complete Hydatidiform Mole Presenting as an Adnexal Tumor in a Postmenopausal Patient.
Stolnicu S, Ilyés Á, Quiñónez E, Nogales FF
International journal of surgical pathology 2014 Aug;22(5):470-2
International journal of surgical pathology 2014 Aug;22(5):470-2
Diagnostic utility of microsatellite genotyping for molar pregnancy testing.
Furtado LV, Paxton CN, Jama MA, Tripp SR, Wilson AR, Lyon E, Jarboe EA, Thaker HM, Geiersbach KB
Archives of pathology & laboratory medicine 2013 Jan;137(1):55-63
Archives of pathology & laboratory medicine 2013 Jan;137(1):55-63
The cyclin-dependent kinase inhibitor p57Kip2 regulates cell cycle exit, differentiation, and migration of embryonic cerebral cortical precursors.
Tury A, Mairet-Coello G, DiCicco-Bloom E
Cerebral cortex (New York, N.Y. : 1991) 2011 Aug;21(8):1840-56
Cerebral cortex (New York, N.Y. : 1991) 2011 Aug;21(8):1840-56
Androgenetic complete mole with trisomy 13: report of a case with microsatellite genotyping and review of the literature.
Dubé V, Chun K, Osborne R, Sherman C, Nofech-Mozes S, Ismiil N, Saad RS, Khalifa MA
Pathology, research and practice 2010 Nov 15;206(11):776-81
Pathology, research and practice 2010 Nov 15;206(11):776-81
MiR-221 and MiR-222 alterations in sporadic ovarian carcinoma: Relationship to CDKN1B, CDKNIC and overall survival.
Wurz K, Garcia RL, Goff BA, Mitchell PS, Lee JH, Tewari M, Swisher EM
Genes, chromosomes & cancer 2010 Jul;49(7):577-84
Genes, chromosomes & cancer 2010 Jul;49(7):577-84
Early molar pregnancy: experience in a large abortion service.
Paul M, Goodman S, Felix J, Lewis R, Hawkins M, Drey E
Contraception 2010 Feb;81(2):150-6
Contraception 2010 Feb;81(2):150-6
Comparison of fluorescence in situ hybridization, p57 immunostaining, flow cytometry, and digital image analysis for diagnosing molar and nonmolar products of conception.
Kipp BR, Ketterling RP, Oberg TN, Cousin MA, Plagge AM, Wiktor AE, Ihrke JM, Meyers CH, Morice WG, Halling KC, Clayton AC
American journal of clinical pathology 2010 Feb;133(2):196-204
American journal of clinical pathology 2010 Feb;133(2):196-204
The villous stromal constituents of complete hydatidiform mole differ histologically in very early pregnancy from the normally developing placenta.
Kim KR, Park BH, Hong YO, Kwon HC, Robboy SJ
The American journal of surgical pathology 2009 Feb;33(2):176-85
The American journal of surgical pathology 2009 Feb;33(2):176-85
Combination of immunohistochemistry and ploidy analysis to assist histopathological diagnosis of molar diseases.
Osterheld MC, Caron L, Chaubert P, Meagher-Villemure K
Clinical medicine. Pathology 2008;1:61-7
Clinical medicine. Pathology 2008;1:61-7
SM22alpha-targeted deletion of bone morphogenetic protein receptor 1A in mice impairs cardiac and vascular development, and influences organogenesis.
El-Bizri N, Guignabert C, Wang L, Cheng A, Stankunas K, Chang CP, Mishina Y, Rabinovitch M
Development (Cambridge, England) 2008 Sep;135(17):2981-91
Development (Cambridge, England) 2008 Sep;135(17):2981-91
Role of DNA content analysis and immunohistochemistry in the evaluation of the risk of unfavourable outcome in Wilms' tumours.
Osterheld MC, Caron L, Meagher-Villemure K
Anticancer research 2008 Mar-Apr;28(2A):751-6
Anticancer research 2008 Mar-Apr;28(2A):751-6
P57KIP2 immunostaining and molecular cytogenetics: combined approach aids in diagnosis of morphologically challenging cases with molar phenotype and in detecting androgenetic cell lines in mosaic/chimeric conceptions.
Hoffner L, Dunn J, Esposito N, Macpherson T, Surti U
Human pathology 2008 Jan;39(1):63-72
Human pathology 2008 Jan;39(1):63-72
Cell cycle regulators show diagnostic and prognostic utility for differentiated thyroid cancer.
Melck A, Masoudi H, Griffith OL, Rajput A, Wilkins G, Bugis S, Jones SJ, Wiseman SM
Annals of surgical oncology 2007 Dec;14(12):3403-11
Annals of surgical oncology 2007 Dec;14(12):3403-11
Glomerular sclerosis in kidneys with congenital nephrotic syndrome (NPHS1).
Kuusniemi AM, Merenmies J, Lahdenkari AT, Holmberg C, Salmela K, Karikoski R, Rapola J, Jalanko H
Kidney international 2006 Oct;70(8):1423-31
Kidney international 2006 Oct;70(8):1423-31
Multiplex short tandem repeat DNA analysis confirms the accuracy of p57(KIP2) immunostaining in the diagnosis of complete hydatidiform mole.
Popiolek DA, Yee H, Mittal K, Chiriboga L, Prinz MK, Caragine TA, Budimlija ZM
Human pathology 2006 Nov;37(11):1426-34
Human pathology 2006 Nov;37(11):1426-34
Diagnostic and pathogenetic significance of increased stromal apoptosis and incomplete vasculogenesis in complete hydatidiform moles in very early pregnancy periods.
Kim MJ, Kim KR, Ro JY, Lage JM, Lee HI
The American journal of surgical pathology 2006 Mar;30(3):362-9
The American journal of surgical pathology 2006 Mar;30(3):362-9
E Proteins and Id2 converge on p57Kip2 to regulate cell cycle in neural cells.
Rothschild G, Zhao X, Iavarone A, Lasorella A
Molecular and cellular biology 2006 Jun;26(11):4351-61
Molecular and cellular biology 2006 Jun;26(11):4351-61
Molecular and immunohistochemical analyses of the focal form of congenital hyperinsulinism.
Suchi M, MacMullen CM, Thornton PS, Adzick NS, Ganguly A, Ruchelli ED, Stanley CA
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 2006 Jan;19(1):122-9
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 2006 Jan;19(1):122-9
Epigenetic down-regulation of CDKN1C/p57KIP2 in pancreatic ductal neoplasms identified by gene expression profiling.
Sato N, Matsubayashi H, Abe T, Fukushima N, Goggins M
Clinical cancer research : an official journal of the American Association for Cancer Research 2005 Jul 1;11(13):4681-8
Clinical cancer research : an official journal of the American Association for Cancer Research 2005 Jul 1;11(13):4681-8
p57KIP2 immunohistochemistry in early molar pregnancies: emphasis on its complementary role in the differential diagnosis of hydropic abortuses.
Merchant SH, Amin MB, Viswanatha DS, Malhotra RK, Moehlenkamp C, Joste NE
Human pathology 2005 Feb;36(2):180-6
Human pathology 2005 Feb;36(2):180-6
Downregulation of Bcl-2 by podocytes is associated with progressive glomerular injury and clinical indices of poor renal prognosis in human IgA nephropathy.
Qiu LQ, Sinniah R, I-Hong Hsu S
Journal of the American Society of Nephrology : JASN 2004 Jan;15(1):79-90
Journal of the American Society of Nephrology : JASN 2004 Jan;15(1):79-90
Downregulation of Bcl-2 by podocytes is associated with progressive glomerular injury and clinical indices of poor renal prognosis in human IgA nephropathy.
Qiu LQ, Sinniah R, I-Hong Hsu S
Journal of the American Society of Nephrology : JASN 2004 Jan;15(1):79-90
Journal of the American Society of Nephrology : JASN 2004 Jan;15(1):79-90
p57(KIP2) immunohistochemical staining of gestational trophoblastic tumours does not identify the type of the causative pregnancy.
Sebire NJ, Rees HC, Peston D, Seckl MJ, Newlands ES, Fisher RA
Histopathology 2004 Aug;45(2):135-41
Histopathology 2004 Aug;45(2):135-41
p57kip2 is useful in the classification and differential diagnosis of complete and partial hydatidiform moles.
Jun SY, Ro JY, Kim KR
Histopathology 2003 Jul;43(1):17-25
Histopathology 2003 Jul;43(1):17-25
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of p57Kip2 was performed by loading 25 µg of Jurkat (lane 1), SH-SY5Y (lane 2) and Neuro-2a (lane 3) cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4ºC overnight. The membrane was probed with a p57Kip2 monoclonal antibody (Product # MA5-11309) at a dilution of 1:50 (Jurkat) and 1:100 (SH-SY5Y and Neuro-2a) overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at ~57 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Formalin-fixed, paraffin-embedded human colon carcinoma stained with p57 antibody using peroxidase-conjugate and AEC chromogen. Note nuclear staining of tumor cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of p57Kip2 was done on SH-SY5Y cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with p57Kip2 Mouse Monoclonal Antibody (MA511309, red histogram) or with mouse isotype control (yellow histogram) at 3-5 ug/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor® 488 Rabbit Anti-Mouse Secondary Antibody (A11059) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10,000 cells were acquired and analyzed for each sample using an Attune® Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of p57Kip2 using p57Kip2 Monoclonal Antibody (Product # MA5-11309) on Native Human LS174T Cells.
