H00000081-D01P

antibody from Abnova Corporation

Targeting: ACTN4 FSGS1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00000081-D01P

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00000081-D01P, RRID:AB_1570889

Product name

ACTN4 purified MaxPab rabbit polyclonal antibody (D01P)

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against a full-length human ACTN4 protein.

Antigen sequence

MVDYHAANQSYQYGPSSAGNGAGGGGSMGDYMAQE
DDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQ
IENIDEDFRDGLKLMLLLEVISGERLPKPERGKMR
VHKINNVNKALDFIASKGVKLVSIGAEEIVDGNAK
MTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQR
KTAPYKNVNVQNFHISWKDGLAFNALIHRHRPELI
EYDKLRKDDPVTNLNNAFEVAEKYLDIPKMLDAED
IVNTARPDEKAIMTYVSSFYHAFSGAQKAETAANR
ICKVLAVNQENEHLMEDYEKLASDLLEWIRRTIPW
LEDRVPQKTIQEMQQKLEDFRDYRRVHKPPKVQEK
CQLEINFNTLQTKLRLSNRPAFMPSEGKMVSDINN
GWQHLEQAEKGYEEWLLNEIRRLERLDHLAEKFRQ
KASIHEAWTDGKEAMLKHRDYETATLSDIKALIRK
HEAFESDLAAHQDRVEQIAAIAQELNELDYYDSHN
VNTRCQKICDQWDALGSLTHSRREALEKTEKQLEA
IDQLHLEYAKRAAPFNNWMESAMEDLQDMFIVHTI
EEIEGLISAHDQFKSTLPDADREREAILAIHKEAQ
RIAESNHIKLSGSNPYTTVTPQIINSKWEKVQQLV
PKRDHALLEEQSKQQSNEHLRRQFASQANVVGPWI
QTKMEEIGRISIEMNGTLEDQLSHLKQYERSIVDY
KPNLDLLEQQHQLIQEALIFDNKHTNYTMEHIRVG
WEQLLTTIARTINEVENQILTRDAKGISQEQMQEF
RASFNHFDKDHGGALGPEEFKACLISLGYDVENDR
QGEAEFNRIMSLVDPNHSGLVTFQAFIDFMSRETT
DTDTADQVIASFKVLAGDKNFITAEELRRELPPDQ
AEYCIARMAPYQGPDAVPGALDYKSFSTALYGESD
L


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of ACTN4 expression in transfected 293T cell line (H00000081-T01) by ACTN4 MaxPab polyclonal antibody.Lane 1: ACTN4 transfected lysate(104.90 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of purified MaxPab rabbit antibody to ACTN4 on HeLa cell. [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between ACTN4 and CTNNB1. HeLa cells were stained with anti-ACTN4 rabbit purified polyclonal 1:1200 and anti-CTNNB1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)