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- Antibody Data
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- References [2]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- NBP1-59254 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-59254, RRID:AB_11039152
- Product name
- Rabbit Polyclonal Connexin 36/GJD2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Exendin-4 overcomes cytokine-induced decreases in gap junction coupling via protein kinase A and Epac2 in mouse and human islets.
Connexin hemichannels contribute to spontaneous electrical activity in the human fetal cortex.
Farnsworth NL, Walter R, Piscopio RA, Schleicher WE, Benninger RKP
The Journal of physiology 2019 Jan;597(2):431-447
The Journal of physiology 2019 Jan;597(2):431-447
Connexin hemichannels contribute to spontaneous electrical activity in the human fetal cortex.
Moore AR, Zhou WL, Sirois CL, Belinsky GS, Zecevic N, Antic SD
Proceedings of the National Academy of Sciences of the United States of America 2014 Sep 16;111(37):E3919-28
Proceedings of the National Academy of Sciences of the United States of America 2014 Sep 16;111(37):E3919-28
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Connexin 36/GJD2 Antibody [NBP1-59254] - Reccomended Titration: 0.2 - 1 ug/ml ELISA Titer: 1:62500 Positive Control: Jurkat cell lysate GJD2 is supported by BioGPS gene expression data to be expressed in Jurkat
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Connexin 36/GJD2 Antibody [NBP1-59254] - Sample Tissue: Human 293T Antibody Dilution: 1.0 ug/ml
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Frozen: Connexin 36/GJD2 Antibody [NBP1-59254] - IHC detection of Connexin 36/GJA9 in 50uM thick section of Mouse Brain. The tissues were pre-fixed overnight in 4% PFA, and primary antibody NBP1-59254 was used at a concentration of 1ug/mL (along with Parvalbumin antibody for double staining) with gentle shaking and overnight incubation at 4C. For the detection of NBP1-59254 primary, anti-rabbit Alexa 555 secondary was employed and images were captured using a confocal microscope at 63X resolution.
