PAB28490

antibody from Abnova Corporation

Targeting: RGS5

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB28490

Provider

Abnova Corporation

Product name

RGS5 polyclonal antibody

Antibody type

Polyclonal

Antigen

Recombinant protein corresponding to amino acids of human RGS5

Reactivity

Human

Host

Rabbit

Antigen sequence

ALPHSCLERAKEIKIKLGILLQKPDSVGDLVIPYN
EKPEKPAKTQKTSLDEALQWRDSLDKLLQNNYGLA
SFKSFLKSEFSEENLEFWIACEDYKKIKSPAKMAE
KAKQIYEEFIQTEAPKEVNIDHFTKDITMKNLVEP


Isotype

IgG

Vial size

100 μl

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: A-431, Lane 4: Liver, Lane 5: Tonsil with RGS5 polyclonal antibody at 1:100-1:250 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS with RGS5 polyclonal antibody (Cat # PAB28490) at 1-4 ug/ml shows positivity in cytoplasm.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human heart muscle with RGS5 polyclonal antibody (Cat # PAB28490) shows moderate cytoplasmic positivity in myocytes at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)