PAB29330

antibody from Abnova Corporation

Targeting: CUL5 VACM-1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB29330

Provider

Abnova Corporation

Product name

CUL5 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant human CUL5.

Antigen sequence

FSLMDKVPNGIEPMLKDLEEHIISAGLADMVAAAE
TITTDSEKYVEQLLTLFNRFSKLVKEAFQDDPRFL
TARDKAYKAVVNDATIFKLELPLKQKGVGLKTQPE
SKCPELLANYCDMLLRK


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Human cell line RT-4; Lane 2: Human cell line U-251MG sp with CUL5 polyclonal antibody (Cat# PAB29330) at 1:100-1:250 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-251 MG with CUL5 polyclonal antibody (Cat# PAB29330) under 1-4 ug/mL working concentration shows positivity in the Golgi apparatus.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human colon with CUL5 polyclonal antibody (Cat# PAB29330) shows strong nuclear and cytoplasmic positivity in glandular cells at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)