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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- PA5-29181 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SLC25A22 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji, mouse brain.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using SLC25A22 Polyclonal Antibody (Product # PA5-29181). Sample (30 µg of whole cell lysate). Lane A: H1299. Lane B: Hela. Lane C: Hep G2. 10% SDS PAGE. SLC25A22 Polyclonal Antibody (Product # PA5-29181) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using SLC25A22 Polyclonal Antibody (Product # PA5-29181). Sample (20 µg of whole cell lysate). Lane A: mouse brain. 10% SDS PAGE. SLC25A22 Polyclonal Antibody (Product # PA5-29181) diluted at 1:10,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of SLC25A22 was performed in A431 cells fixed in 4% paraformaldehyde at RT for 15 min. Green: SLC25A22 Polyclonal Antibody (Product # PA5-29181) diluted at 1:200. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of SLC25A22 was performed in paraffin-embedded human colon carcinoma tissue using SLC25A22 Polyclonal Antibody (Product # PA5-29181) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using SLC25A22 (Product # PA5-29181) antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
