25415-1-AP
Immunohistochemistry of paraffin-embedded mouse embryo tissue slide using 25415-1-AP ( SOX15 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
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PA5-80100
Western blot analysis of TCP1 using TCP1 Polyclonal Antibody (Product # PA5-80100). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 µg of sample under reducing conditions. Lane 1: rat heart tissue lysates. Lane 2: rat ovary tissue lysates. Lane 3: rat brain tissue lysates. Lane 4: rat lung tissue lysates. Lane 5: mouse heart tissue lysates. Lane 6: mouse ovary tissue lysates. Lane 7: mouse brain tissue lysates. Lane 8: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with TCP1 Polyclonal Antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5,000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TCP1 at approximately 60 kDa. The expected band size for TCP1 is at 60 kDa.
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LS-C782328
IHC testing of FFPE human spleen tissue with CD22 antibody (clone BLCAM/1795). HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
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ABIN462190
IHC
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720087
Figure 3 Histone acetylation and methylation in HDAC1 wt and HDAC1 dn mESCs induced into cardiomyocytes and treated with HDACi. The level of H3K9ac, H3K9me3, H4ac, H4K20ac, pan-acetylated lysines (K-ac), and alpha-actinin in ( A ) HDAC1 wt mESCs and ( B ) HDAC1 dn mESCs. In three biological replicates, Western blots were performed on one gel. For the data presented in panel A or B, the gel was separated by Photoshop to show samples that were compared in one relevant subset. Data on histone levels were normalized to the level of histone H3 and non-histone proteins were normalized and quantified to the level of GAPDH ( C ). In wt and HDAC1 dn non-treated cells and in TSA-, SAHA-, or VPA-treated mESCs, panel ( Ca ) shows the levels of H4ac, ( Cb ) shows H4K20ac, and ( Cc ) shows the levels of alpha-actinin. The total protein levels were measured using a uQuant spectrophotometer for each sample, and an identical protein amount was loaded on the gels. In panel ( A , B ), the levels of histone markers are also shown for embryonic hearts (e15). Quantification of the protein levels in panel ( C ) was performed using ImageJ software (NIH, freeware). Statistical analyses were performed using Student's t -test; asterisks (*) in panel ( Ca - c ) show statistically significant differences at p
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ABIN2987909
IHC
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TA505044
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ZBED1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505044)
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56-9003-82
Immunohistochemical analysis of PanCK was performed on FFPE human tonsil tissue. To expose the target protein, HIER was performed on de-paraffinized sections using BOND Epitope Retrieval Solution 2 (pH 9) for 10 mins, followed by a 5-min cool down and a 5-min wash with ddH2O. Tissues were permeabilized with 0.1% Triton X-100 in 1X PBS for 30 mins and blocked with 3% BSA/5% normal goat serum/1X PBS for 1 hr at RT. Following the removal of the blocking solution, tissues were probed with Pan Cytokeratin Monoclonal Antibody (AE1/AE3), Alexa Fluor™ 700 (Product # 56-9003-82) at 2 µg/mL (left) or Cytokeratin Pan Type I/II Antibody Cocktail (AE1/AE3), eBioscience™ (Product # MA5-13156) at 0.5 µg/mL (right), respectively, in blocking solution for one hr at RT in a humidified chamber. Tissues were then washed three times, 5 mins each, in 1X PBS. Detection of the unconjugated primary antibody was performed using Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 594 (Product # A32742) at a dilution of 1:2,000 in blocking solution for one hr at RT, followed by three, 5-minute washes in 1X PBS. Sections were stained with DAPI (Product # 62247) at 1 µg/mL diluted in 1X PBS for 5 minutes at RT, washed twice in 1X PBS followed by a final rinse in ddH2O. Tissues were mounted with ProLong™ Glass Antifade Mountant (Product # P36982) and images were captured on EVOS™ M7000 Imaging System (Product # AMF7000) at 20X magnification.
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FAB11381A-025
Detection of Siglec-7/CD328 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human NCAM-1/CD56 PE-conjugated Monoclonal Antibody (Catalog # FAB2408P) and either (A) Mouse Anti-Human Siglec-7/CD328 APC-conjugated Monoclonal Antibody (Catalog # FAB11381A) or (B) Mouse IgG₁ Allophycocyanin Isotype Control (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.
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BS-0623R
Image provided by One World Lab validation program. HEK293 cells probed with Rabbit Anti-Cyclin D1 Polyclonal Antibody (bs-0623R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
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