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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunoprecipitation [2]
- Immunohistochemistry [1]
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- Product number
- NB100-40832 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-40832, RRID:AB_2213361
- Product name
- Rabbit Polyclonal USP36 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: USP36 Antibody [NB100-40832] - Detection of Human USP36/DUB1 by Western Blot. Samples: Whole cell lysate (15 and 50 ug) from HeLa and (50 ug) 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-USP36/DUB1 antibody NB100-40832 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: USP36 Antibody [NB100-40832] - Whole cell lysate (50 ug) from HeLa, Hep-G2, and U2OS cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-USP36/DUB1 antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: USP36 Antibody [NB100-40832] - Detection of Human USP36/DUB1 on HeLa whole cell lysate using NB100-40832. USP36/DUB1 was also immunoprecipitated by another rabbit anti- USP36/DUB1 antibody and NB100-40831.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: USP36 Antibody [NB100-40832] - Detection of human USP36/DUB1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-USP36/DUB1 antibody NB100-40832 used for IP at 6 ug per reaction. USP36/DUB1 was also immunoprecipitated by another rabbit anti-USP36/DUB1 antibody. For blotting immunoprecipitated USP36/DUB1, NB100-40832 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: USP36 Antibody [NB100-40832] - Section of human colon carcinoma. Antibody: Affinity purified rabbit anti- USP36/DUB1 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB
