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A portal for validated antibodies

Antibodypedia scores antibodies to guide researchers to choose an appropriate antibody for a particular application. The resource contains information about more than four million publicly available research antibodies towards over 19,000 human protein targets from more than 100 providers.

Use "Search for" to find validated antibodies against your target protein for a particular application! The antibodies are scored using the validation principles outlined by the International Working Group for Antibody Validation and we encourage feedback from researcher by submitting validation data for a particular antibody.

Content updated 2024-10-25
5365912 reviewed antibodies from 105 providers,
covering gene-products encoded by 19163 genes (approximately 95% of all human genes).
Primary data available for 2745901 experiments.

Featured Validations

  • ABIN5579321
    WB
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  • PA5-28316
    Western Blot using GIT1 Polyclonal Antibody (Product # PA5-28316). Sample (30 µg of whole cell lysate). A: Hep G2. 7.5% SDS PAGE. GIT1 Polyclonal Antibody (Product # PA5-28316) diluted at 1:2,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
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  • 34-2000
    NULL
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  • ABIN2156859
    Image(s): Western Blotting
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  • AF5589
    Detection of Human/Mouse LRRTM2 by Western Blot. Western blot shows lysates of human brain cortex and mouse brain tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse LRRTM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5589) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for LRRTM2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
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  • NBP2-82325-0.1ml
    Western Blot: RAB37 Antibody [NBP2-82325] - WB Suggested Anti-RAB37 Antibody Titration: 0.2-1 ug/ml. Positive Control: Transfected 293T
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  • 5741-MSM2X-P1
    Formalin-fixed, paraffin-embedded human Parathyroid stained with PTH Mouse Monoclonal Antibody (SPM604).
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  • 16-0049-025
    NULL
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  • PA5-17850
    Immunofluorescence analysis of Phospho-EGFR pTyr845 was performed using 90% confluent log phase A-431 cells treated with 200 ng/mL of EGF for 10 minutes. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Phospho-EGFR (Tyr845) Rabbit Polyclonal Antibody (Product # PA5-17850) at 1:250 dilution in 0.1% BSA and incubated overnight at 4 degree Celsius and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing membranous localization. Panel e represents cells treated with antagonist, Afatinib (1µM for 6hrs) followed by EGF (200 ng/mL for 10 minutes), showing reduced Phospho-EGFR staining. Panel f shows untreated cells with no signal. Panel g represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
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  • TA505376
    HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CMPK1 (RC204856, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CMPK1.
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