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A portal for validated antibodies

Antibodypedia scores antibodies to guide researchers to choose an appropriate antibody for a particular application. The resource contains information about more than four million publicly available research antibodies towards over 19,000 human protein targets from more than 100 providers.

Use "Search for" to find validated antibodies against your target protein for a particular application! The antibodies are scored using the validation principles outlined by the International Working Group for Antibody Validation and we encourage feedback from researcher by submitting validation data for a particular antibody.

Content updated 2024-10-25
5365912 reviewed antibodies from 105 providers,
covering gene-products encoded by 19163 genes (approximately 95% of all human genes).
Primary data available for 2745901 experiments.

Featured Validations

  • ABIN2624658
    Image(s): Immunohistochemistry
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  • TA500816
    Anti-TTLL12 mouse monoclonal antibody (TA500816) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TTLL12(RC200841).
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  • LS-C756385
    IHC analysis of C Reactive Protein using anti-C Reactive Protein antibody. C Reactive Protein was detected in paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-C Reactive Protein Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
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  • F49791
    IHC analysis of FFPE human brain tissue stained with PAX4 antibody
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  • TA803488
    Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-RBFOX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803488)
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  • MA5-17278
    Immunofluorescence analysis of HMGB1 was performed using 90% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with HMGB1 Monoclonal Antibody (GT348) (Product # MA5-17278) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
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  • PA5-105399
    Immunohistochemistry analysis of paraffin-embedded Phospho-LAT (Tyr45) in human kidney tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-LAT (Tyr45) polyclonal antibody (Product # PA5-105399) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.
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  • PA5-78843
    Western blot analysis of ATP7B in Lane 1: rat liver tissue lysate, Lane 2: rat brain tissue lysate, Lane 3: SMMC whole cell lysate, Lane 4: NEURO whole cell lysate using 40-50 µg per well. Sample was incubated with ATP7B (Product # PA5-78843) at a dilution of 0.5 µg/mL.
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  • TA506386
    CD163 Luminex with 3B4 Capture (TA506386) and 2C7 Detection (TA506384) Antibodies. Substrate used: full length HEK293 cells expressed recombinant protein of human CD163 (TP308557).
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  • HPA015625
    Western blot analysis in control (vector only transfected HEK293T lysate) and TIMD4 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY408645).
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