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A portal for validated antibodies

Antibodypedia scores antibodies to guide researchers to choose an appropriate antibody for a particular application. The resource contains information about more than four million publicly available research antibodies towards over 19,000 human protein targets from more than 100 providers.

Use "Search for" to find validated antibodies against your target protein for a particular application! The antibodies are scored using the validation principles outlined by the International Working Group for Antibody Validation and we encourage feedback from researcher by submitting validation data for a particular antibody.

Content updated 2024-10-25
5365912 reviewed antibodies from 105 providers,
covering gene-products encoded by 19163 genes (approximately 95% of all human genes).
Primary data available for 2745901 experiments.

Featured Validations

  • CF807788
    Western blot analysis of extracts (35 µg) from 9 different cell lines by using anti-MSTN monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human). (1:200)
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  • LS-C335585
    Immunohistochemistry of paraffin-embedded Rat kidney tissue.
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  • 685102
    20 or 50 ng of human FGF-3 recombinant protein was resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-human FGF-3 (clone W15075A) antibody. Proteins were visualized using an HRP Goat anti-rat IgG antibody and chemiluminescence detection.
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  • TA809264S
    Western blot analysis of extracts (35 µg) from 3 different cell lines by using anti-GC monoclonal antibody (HeLa: human; 293T: human; T47D: human).(1:500)
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  • TA804766S
    Immunohistochemical staining of paraffin-embedded human colon tissue within the normal limits using anti-RSPO1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804766)
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  • LS-C759204
    GFAP Antibody in Western Blot (WB)
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  • PA5-58923
    Western blot analysis of TBC1D30 in Lane 1: Marker (kDa) 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp. Samples were probed using a TBC1D30 Polyclonal Antibody (Product # PA5-58923).
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  • PAB13327
    Western blot analysis of SLITRK1 in human brain tissue lysate with SLITRK1 polyclonal antibody (Cat # PAB13327) at (A) 1 and (B) 2 ug/mL .
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  • 67296-1-IG
    Various lysates were subjected to SDS PAGE followed by western blot with 67296-1-Ig (DOCK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
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  • A304-152A
    Detection of human GAS2L3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-GAS2L3 antibody A304-152A (lot A304-152A-1) used for IP at 6 µg/mg lysate. GAS2L3 was also immunoprecipitated by rabbit anti-GAS2L3 antibody A304-153A. For blotting immunoprecipitated GAS2L3, A304-153A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
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