15659-1-AP

antibody from Invitrogen Antibodies

Targeting: PIWIL1 CT80.1, HIWI, PIWI

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

15659-1-AP

Provider

Invitrogen Antibodies

Product name

PIWIL1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MTGRARARA RGRARGQETA QLVGSTASQQ PGYIQPRPQP PPAEGELFGR GRQRGTAGGT AKSQGLQISA GFQELSLAER GGRRRDFHDL GVNTRQNLDH VKESKTGSSG IIVRLSTNHF RLTSRPQWAL YQYHIDYNPL MEARRLRSAL LFQHEDLIGK CHAFDGTILF LPKRLQQKVT EVFSKTRNGE DVRITITLTN ELPPTSPTCL QFYNIIFRRL LKIMNLQQIG RNYYNPNDPI DIPSHRLVIW PGFTTSILQY ENSIMLCTDV SHKVLRSETV LDFMFNFYHQ TEEHKFQEQV SKELIGLVVL TKYNNKTYRV DDIDWDQNPK STFKKADGSE VSFLEYYRKQ Y (1-350 aa encoded by BC028581)

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.19 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human testis tissue were subjected to SDS PAGE followed by western blot with 15659-1-AP (PIWIL1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse testis tissue were subjected to SDS PAGE followed by western blot with 15659-1-AP (PIWIL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human testis tissue were subjected to SDS PAGE followed by western blot with 15659-1-AP ( PIWIL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells using 15659-1-AP (PIWIL1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human prostate cancer tissue slide using 15659-1-AP (PIWIL1 Antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human prostate cancer tissue slide using 15659-1-AP (PIWIL1 Antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 15659-1-AP (PIWIL1 Antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 15659-1-AP (PIWIL1 Antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human testis tissue slide using 15659-1-AP (PIWIL1 Antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human testis tissue slide using 15659-1-AP ( PIWIL1 Antibody) at dilution of 1:50 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-PIWIL1 (IP:15659-1-AP, 4ug; Detection:15659-1-AP 1:500) with mouse testis tissue lysate 2400ug.