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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunohistochemistry [1]
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- Product number
- H00022846-M05 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00022846-M05, RRID:AB_1716896
- Product name
- VASH1 monoclonal antibody (M05), clone 4A3
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a partial recombinant VASH1.
- Antigen sequence
GGKKVAGGGSSGATPTSAAATAPSGVRRLETSEGT
SAQRDEEPEEEGEEDLRDGGVPFFVNRGGLPVDEA
TWERMWKHVAKIHPDGEKVAQRIRGATD- Isotype
- IgG
- Antibody clone number
- 4A3
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Vasohibin-1 increases the malignant potential of colorectal cancer and is a biomarker of poor prognosis.
Upregulation of vasohibin-1 expression with angiogenesis and poor prognosis of hepatocellular carcinoma after curative surgery.
Kitajima T, Toiyama Y, Tanaka K, Saigusa S, Kobayashi M, Inoue Y, Mohri Y, Kusunoki M
Anticancer research 2014 Oct;34(10):5321-9
Anticancer research 2014 Oct;34(10):5321-9
Upregulation of vasohibin-1 expression with angiogenesis and poor prognosis of hepatocellular carcinoma after curative surgery.
Wang Q, Tian X, Zhang C, Wang Q
Medical oncology (Northwood, London, England) 2012 Dec;29(4):2727-36
Medical oncology (Northwood, London, England) 2012 Dec;29(4):2727-36
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- VASH1 monoclonal antibody (M05), clone 4A3. Western Blot analysis of VASH1 expression in HepG2(Cat # L019V1 ).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged VASH1 is 0.03 ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to VASH1 on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 3 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
