Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- 17701-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CDCA2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: ATSDEDPNT NIMNINENKN IPKAKNKSES ENEPKAGTDS PVSCASITEE RVASDSPKPA LTLQQGQEFS AGGQNAENLC QFFKISPDLN IKCERKDDFL GAAEGKLQCN RLMPNSQKDC HCLGDVLIEN TKESKSQSED LGRKPMESSS VVSCRDRKDR RRSMCYSDGR SLHLEKNGNH TPSSSVGSSV EISLENSELF KDLSDAIEQT FQRRNSETKV RRSTRLQKDL ENEGLVWISL PLPSTSQKAK RRTICTFDSS GFESMSPIKE TVSSRQKPQM APPVSDPENS QGPAAGSSDE PGKRRKSFCI STLANTKATS QFKGYRRRSS LNGKGESSLT ALERIEHNGE RKQ (512-863 aa encoded by BC036214)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.31 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 17701-1-AP (CDCA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MCF7 cells were subjected to SDS PAGE followed by western blot with 17701-1-AP (CDCA2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 17701-1-AP (CDCA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( 4% PFA) fixed HepG2 cells using 17701-1-AP (CDCA2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-CDCA2 (IP:17701-1-AP, 4ug; Detection:17701-1-AP 1:800) with Jurkat cells lysate 2800ug.