NBP2-36338

antibody from Novus Biologicals

Targeting: EMILIN1 DKFZp586M121, gp115

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-36338

Provider

Novus Biologicals

Product name

Rat Monoclonal EMILIN1 Antibody

Antibody type

Monoclonal

Description

Protein G purified.

Reactivity

Mouse

Host

Rat

Isotype

IgG

Vial size

0.1 mg

Concentration

LYOPH

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: EMILIN1 Antibody (C11A8) [NBP2-36338] - Western blot on recombinant protein obtained from supernatant of cells expressing human EMILIN1 (hE1), mouse EMILIN1 (mE1), mouse EMILIN2 (mE2).

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Frozen: EMILIN1 Antibody (C11A8) [NBP2-36338] - EMILIN1 is expressed in association with lymphatic vessels. Cryostat sections of normal mouse tissues stained with anti-EMILIN1 (green) antibodies. In all mouse tissues and organs examined, EMILIN1 was uniformly distributed in the stroma. In the skin, EMILIN1 staining colocalizes with LYVE-1-positive lymphatic vessels surrounding hair follicles. In the small intestine, EMILIN1 colocalizes with LYVE-1-positive lacteals and submucosal lymphatic vessels. At higher magnification, in the lung and lymph nodes, it is more evident that EMILIN1 is distributed at the abluminal surfaces of LECs . In the lymph node, EMILIN1-positive fibers connecting LECs to the surrounding ECM are evident.