12075-1-AP

antibody from Invitrogen Antibodies

Targeting: PYGB

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

12075-1-AP

Provider

Invitrogen Antibodies

Product name

PYGB Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: LKFSAFLEK EYKVKINPSS MFDVHVKRIH EYKRQLLNCL HVVTLYNRIK RDPAKAFVPR TVMIGGKAAP GYHMAKLIIK LVTSIGDVVN HDPVVGDRLK VIFLENYRVS LAEKVIPAAD LSQQISTAGT EASGTGNMKF MLNGALTIGT MDGANVEMAE EAGAENLFIF GLRVEDVEAL DRKGYNAREY YDHLPELKQA VDQISSGFFS PKEPDCFKDI VNMLMHHDRF KVFADYEAYM QCQAQVDQLY RNPKEWTKKV IRNIACSGKF SSDRTITEYA REIWGVEPSD LQIPPPNIPR D (544-843 aa encoded by BC017045)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.19 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human brain tissue were subjected to SDS PAGE followed by western blot with 12075-1-AP (PYGB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Rat brain tissue were subjected to SDS PAGE followed by western blot with 12075-1-AP (PYGB Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded rat brain tissue slide using 12075-1-AP (PYGB antibody) at dilution of 1:200 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded rat brain tissue slide using 12075-1-AP (PYGB antibody) at dilution of 1:200 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded mouse heart tissue slide using 12075-1-AP (PYGB antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded mouse heart tissue slide using 12075-1-AP (PYGB antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-PYGB (IP:12075-1-AP, 4ug; Detection:12075-1-AP 1:500) with mouse brain tissue lysate 4000ug.