Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [2]
- Other assay [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-28022 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GPBB Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2, Mouse brain.
- Concentration
- 0.36 mg/mL
Submitted references Breast cancers utilize hypoxic glycogen stores via PYGB, the brain isoform of glycogen phosphorylase, to promote metastatic phenotypes.
Altemus MA, Goo LE, Little AC, Yates JA, Cheriyan HG, Wu ZF, Merajver SD
PloS one 2019;14(9):e0220973
PloS one 2019;14(9):e0220973
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GPBB using 30 µg of HepG2 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a GPBB polyclonal antibody (Product # PA5-28022) at a dilution of 1:2000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of GPBB was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a GPBB Polyclonal Antibody (Product # PA5-28022) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using GPBB Polyclonal Antibody (Product # PA5-28022). Sample (50 µg of whole cell lysate). Lane A: Mouse brain. 7.5% SDS PAGE. GPBB Polyclonal Antibody (Product # PA5-28022) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- GPBB Polyclonal Antibody detects GPBB protein at cytosol on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. GPBB Polyclonal Antibody (Product # PA5-28022) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- GPBB Polyclonal Antibody detects PYGB protein at cytosol on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. GPBB Polyclonal Antibody (Product # PA5-28022) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig 3 Glycogen phosphorylase brain isoform knockdown inhibits glycogen utilization in breast cancer cells. (A) Western blot of liver and brain glycogen phosphorylase (PYGL and PYGB) in wild-type, either empty vector, shPYGL and shPYGB transduced MDA-MB-231, MCF-7, and MCF-10A cells. Actin is shown as a loading control(B-D) Glycogen levels normalized to protein in lysate after 24h in hypoxia (Hypoxic Control) or 24h in hypoxia with subsequent 24h in normoxia (Normoxic Exposure) for wild-type, empty vector, shPYGL, and shPYGB in (B) MDA-MB-231, (C) MCF-7, and (D) MCF-10A cells, respectively. Error bars represent SEM of n = 3. *p
