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LearnAAR-011-200UL
antibody from Invitrogen Antibodies
Targeting: AGTR1
AG2S, AGTR1A, AGTR1B, AT1, AT1B, AT2R1, AT2R1A, AT2R1B, HAT1R
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- AAR-011-200UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Angiotensin II Receptor Type-1 (extracellular) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Reconstitution: 1 X 25 µL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 X g 5 min).
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µL
- Concentration
- 0.7 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysisof rat liver (lanes 1 and 3) and rat kidney (lanes 2 and 4) membranes: - 1,2. Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody (#AAR-011), (1:200).3,4. Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody , preincubated with Angiotensin II Receptor Type-1 (extracellular) Blocking Peptide (#BLP-AR011).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysisof mouse kidney (lanes 1 and 3) and mouse heart (lanes 2 and 4) membranes: - 1,2. Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody (#AAR-011), (1:500).3,4. Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody , preincubated with Angiotensin II Receptor Type-1 (extracellular) Blocking Peptide (#BLP-AR011).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysisof rat liver (lanes 1 and 3) and rat kidney (lanes 2 and 4) membranes: - 1,2. Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody (#AAR-011), (1:200).3,4. Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody , preincubated with Angiotensin II Receptor Type-1 (extracellular) Blocking Peptide (#BLP-AR011).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysisof mouse kidney (lanes 1 and 3) and mouse heart (lanes 2 and 4) membranes: - 1,2. Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody (#AAR-011), (1:500).3,4. Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody , preincubated with Angiotensin II Receptor Type-1 (extracellular) Blocking Peptide (#BLP-AR011).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of AGTR1 in rat C6 glioma cells - Cell surface detection of AGTR1 in live intact rat C6 glioma cells. A. Cells were stained using Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody (#AAR-011) (1:100), followed by goat Anti-rabbit-AlexaFluor-555 secondary Antibody . B. Live intact C6 cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression ofAngiotensin II Receptor Type-1 in mouse cerebellum - Immunohistochemical staining of mouse cerebellum using Anti-Angiotensin II ReceptorType-1 (extracellular) Antibody (#AAR-011).A.Mouse Anti-Parvalbumin (red) is detected in the Purkinje layer. B. In the same section, AT1 receptor (green) is also present in the Purkinje layer. Arrows point at AT1 receptor immunoreactive cells. Merge of A and B panelsreveals partial co-localization.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cell surface detection of AGTR1 by indirect flow cytometry in live intact human THP-1 monocytic leukemia cells: - (black line) cells. (red) Cells + goat- Anti-rabbit-FITC. (green) Cells + Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody (#AAR-011), (2.5μg) + goat- Anti-rabbit-FITC.
