AAR-011-200UL

antibody from Invitrogen Antibodies

Targeting: AGTR1 AG2S, AGTR1A, AGTR1B, AT1, AT1B, AT2R1, AT2R1A, AT2R1B, HAT1R

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AAR-011-200UL

Provider

Invitrogen Antibodies

Product name

Angiotensin II Receptor Type-1 (extracellular) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

200 µL

Concentration

0.7 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysisof rat liver (lanes 1 and 3) and rat kidney (lanes 2 and 4) membranes: - 1,2. Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody (#AAR-011), (1:200).3,4. Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody , preincubated with Angiotensin II Receptor Type-1 (extracellular) Blocking Peptide (#BLP-AR011).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysisof mouse kidney (lanes 1 and 3) and mouse heart (lanes 2 and 4) membranes: - 1,2. Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody (#AAR-011), (1:500).3,4. Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody , preincubated with Angiotensin II Receptor Type-1 (extracellular) Blocking Peptide (#BLP-AR011).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysisof rat liver (lanes 1 and 3) and rat kidney (lanes 2 and 4) membranes: - 1,2. Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody (#AAR-011), (1:200).3,4. Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody , preincubated with Angiotensin II Receptor Type-1 (extracellular) Blocking Peptide (#BLP-AR011).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysisof mouse kidney (lanes 1 and 3) and mouse heart (lanes 2 and 4) membranes: - 1,2. Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody (#AAR-011), (1:500).3,4. Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody , preincubated with Angiotensin II Receptor Type-1 (extracellular) Blocking Peptide (#BLP-AR011).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Expression of AGTR1 in rat C6 glioma cells - Cell surface detection of AGTR1 in live intact rat C6 glioma cells. A. Cells were stained using Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody (#AAR-011) (1:100), followed by goat Anti-rabbit-AlexaFluor-555 secondary Antibody . B. Live intact C6 cells.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Expression ofAngiotensin II Receptor Type-1 in mouse cerebellum - Immunohistochemical staining of mouse cerebellum using Anti-Angiotensin II ReceptorType-1 (extracellular) Antibody (#AAR-011).A.Mouse Anti-Parvalbumin (red) is detected in the Purkinje layer. B. In the same section, AT1 receptor (green) is also present in the Purkinje layer. Arrows point at AT1 receptor immunoreactive cells. Merge of A and B panelsreveals partial co-localization.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Cell surface detection of AGTR1 by indirect flow cytometry in live intact human THP-1 monocytic leukemia cells: - (black line) cells. (red) Cells + goat- Anti-rabbit-FITC. (green) Cells + Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody (#AAR-011), (2.5μg) + goat- Anti-rabbit-FITC.