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LearnADI-905-743-100
antibody from Enzo Life Sciences
Targeting: AGTR1
AG2S, AGTR1A, AGTR1B, AT1, AT1B, AT2R1, AT2R1A, AT2R1B, HAT1R
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [5]
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- Product number
- ADI-905-743-100 - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#ADI-905-743-100, RRID:AB_2038802
- Product name
- Angiotensin II receptor AT1, pAb
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide affinity purified. Liquid. In PBS containing 50% glycerol and 0.01% sodium azide.
Submitted references Immunohistochemical detection of angiotensin II receptors in mouse cerebellum and adrenal gland using "in vivo cryotechnique".
Huang Z, Ohno N, Terada N, Saitoh Y, Chen J, Ohno S
Histochemistry and cell biology 2013 Oct;140(4):477-90
Histochemistry and cell biology 2013 Oct;140(4):477-90
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Supportive validation
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Western blot analysis: MW marker (1) and 20µg of kidney membrane extract (2) probed with Angiotensin II Receptor (AT1) pAb at 3 µg/ml.
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis: Rat brain nucleus tractis solitarus fixed with 100% methanol and stained with propidium iodide (red) and Angiotensin II Receptor (AT1) pAb at 1:1000 (green).
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Cellular ELISA: HEK293 cells expressing AT1 Receptor were treated with indicated concentrations of agonist (Angiotensin II) and probed with Angiotensin II Receptor (AT1) pAb (1:1000 of a stock solution of 1µg/µL) by ELISA. Data from vehicle treated cells were taken as 100%. Results are the mean ± SEM (n=3).
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Membrane ELISA: Lewis rat kidney membranes (5 µg/well) were treated with 1 µM concentrations of agonist (Angiotensin II) and probed with Angiotensin II Receptor (AT1) pAb (1:250 to 1:2000 of a 1 µg/µL stock solution) by ELISA. Data from vehicle treated cells were taken as 100%. Results are the mean ± SEM (n=2).
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Membrane ELISA: Lewis rat kidney membranes (5µg/well) were treated with indicated concentrations of agonist (Angiotensin II) and antagonist (Losartan) and probed with Angiotensin II Receptor (AT1) pAb (1:1000 of a stock solution of 1µg/µL) by ELISA. Data from vehicle treated cells were taken as 100%. Results are the mean ± SEM (n=3).
