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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- AB10032-200 - Provider product page
- Provider
- SICGEN
- Proper citation
- SICGEN Cat#AB10032-200, RRID:AB_2333165
- Product name
- Anti-Rab3
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody to mouse Rab5. Rab5 belongs to the small GTPase superfamily, Rab family. Rab5a is an Early Endosome Marker and functions as a key regulator of vesicular trafficking during early endocytosis.
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Donkey, Feline, Goat, Guinea Pig, Hamster, Horse, Porcine, Rabbit, Sheep, Simian, Other
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 600 µg
- Concentration
- 3 mg/ml
- Storage
- Store at -20 C for long-term storage. Store at 2-8 C for up to one month.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references A Rab3a-dependent complex essential for lysosome positioning and plasma membrane repair.
Encarnação M, Espada L, Escrevente C, Mateus D, Ramalho J, Michelet X, Santarino I, Hsu VW, Brenner MB, Barral DC, Vieira OV
The Journal of cell biology 2016 Jun 20;213(6):631-40
The Journal of cell biology 2016 Jun 20;213(6):631-40
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Supportive validation
- Submitted by
- SICGEN (provider)
- Main image
- Experimental details
- Cell lysates at 100 µg per lane
- Sample type
- AtT-20
- Primary Ab dilution
- 1/500
- Conjugate
- Unconjugated
- Secondary Ab
- Secondary Ab
- Secondary Ab dilution
- 1/10,000
Supportive validation
- Submitted by
- SICGEN (provider)
- Main image
- Experimental details
- Cell were fixed with methanol
- Sample type
- B6-RPE07
- Primary Ab dilution
- 1/50
- Conjugate
- Unconjugated
