Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- TA503920 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA503920, RRID:AB_11126353
- Product name
- PTK7 (CCK4) mouse monoclonal antibody, clone OTI2E7 (formerly 2E7)
- Antibody type
- Monoclonal
- Description
- PTK7 (CCK4) mouse monoclonal antibody, clone OTI2E7 (formerly 2E7)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- PTK7
- Isotype
- IgG
- Antibody clone number
- OTI2E7
- Vial size
- 100 µl
- Concentration
- NULL
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PTK7 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PTK7 (RC223416, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTK7.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-PTK7 mouse monoclonal antibody (TA503920) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PTK7(RC223416).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC223416 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PTK7 antibody(TA503920), and then analyzed by flow cytometry.
- Validation comment
- FC