AF4686
antibody from Novus Biologicals
Targeting: FGF1
AFGF, ECGF, ECGF-beta, ECGFA, ECGFB, FGF-alpha, FGFA, GLIO703, HBGF1
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- AF4686 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Sheep Polyclonal FGF acidic/FGF1 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse FGF acidic/FGF1 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human FGF acidic is observed, and less than 1% cross-reactivity with recombinant mouse (rm) FGF7, rmFGF21, and rmFGF23 is observed.
- Reactivity
- Mouse
- Host
- Sheep
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Mouse FGF acidic by Western Blot. Western blot shows lysates of mouse heart tissue and mouse kidney tissue. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Mouse FGF acidic Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4686) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for FGF acidic at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by FGF acidic and Neutralization by Mouse FGF acidic Antibody. Recombinant Mouse FGF acidic (Catalog # 4686-FA) induces proliferation in the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse FGF acidic (1 ng/mL) is neutralized (green line) by increasing concentrations of Sheep Anti-Mouse FGF acidic Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF4686). The ND50 is typically 0.1-0.6 ug/mL.