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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- MAB2091 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB2091, RRID:AB_1678577
- Product name
- NEDD9 monoclonal antibody, clone 14A11
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against partial recombinant NEDD9.
- Isotype
- IgG
- Antibody clone number
- 14A11
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Crk-associated substrate (Cas) family member, NEDD9, is regulated in human neuroblastoma cells and in the embryonic hindbrain by all-trans retinoic acid.
Regulation of HEF1 expression and phosphorylation by TGF-beta 1 and cell adhesion.
Human enhancer of filamentation 1, a novel p130cas-like docking protein, associates with focal adhesion kinase and induces pseudohyphal growth in Saccharomyces cerevisiae.
Merrill RA, See AW, Wertheim ML, Clagett-Dame M
Developmental dynamics : an official publication of the American Association of Anatomists 2004 Nov;231(3):564-75
Developmental dynamics : an official publication of the American Association of Anatomists 2004 Nov;231(3):564-75
Regulation of HEF1 expression and phosphorylation by TGF-beta 1 and cell adhesion.
Zheng M, McKeown-Longo PJ
The Journal of biological chemistry 2002 Oct 18;277(42):39599-608
The Journal of biological chemistry 2002 Oct 18;277(42):39599-608
Human enhancer of filamentation 1, a novel p130cas-like docking protein, associates with focal adhesion kinase and induces pseudohyphal growth in Saccharomyces cerevisiae.
Law SF, Estojak J, Wang B, Mysliwiec T, Kruh G, Golemis EA
Molecular and cellular biology 1996 Jul;16(7):3327-37
Molecular and cellular biology 1996 Jul;16(7):3327-37
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blotting using NEDD9 monoclonal antibody, clone 14A11 (Cat # MAB2091) shows detection of NEDD9 present in MCF-7 cells induced to express NEDD9 by tetracycline removal (right lane).See Pugacheva et al for details.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence microscopyusing NEDD9 monoclonal antibody, clone 14A11 (Cat # MAB2091) shows detectionof NEDD9 localized at the centrosome (bright dots) and focal adhesion sites.The antibody was used at a 1:100 dilution.Personal Communication. Elena Pugacheva, FoxChase Cancer Center, Philadelphia, PA.
- Validation comment
- Immunofluorescence
