PA5-102624

antibody from Invitrogen Antibodies

Targeting: NEDD9 CAS-L, CASS2, HEF1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-102624

Provider

Invitrogen Antibodies

Product name

HEF1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20°C

References

Submitted references

High neural precursor cell-expressed developmentally down-regulated 9 expression is a poor prognostic indicator for osteosarcoma.

Feng J, Gu Y, Zhou L, Rui G, Liu W
Translational cancer research 2020 Nov;9(11):7196-7204

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of HEF1 in 293 cell lysate (Lane 1: treated with the antigen-specific peptide). Samples were incubated with HEF1 polyclonal antibody (Product # PA5-102624).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of HEF1 in rat brain lysate. Samples were incubated with HEF1 polyclonal antibody (Product # PA5-102624).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of paraffin-embedded HEF1 in mouse lung tissue. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with HEF1 polyclonal antibody (Product # PA5-102624) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 1 Immunohistochemistry (IHC) for neural precursor cell-expressed developmentally down-regulated 9 (NEDD9) in osteosarcoma. Osteosarcoma tissue was stained with an anti-NEDD9 antibody as negative staining (A,B). Low-level staining in osteosarcoma tissue (C,D). High-level staining in osteosarcoma tissue (E,F).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 1 Immunohistochemistry (IHC) for neural precursor cell-expressed developmentally down-regulated 9 (NEDD9) in osteosarcoma. Osteosarcoma tissue was stained with an anti-NEDD9 antibody as negative staining (A,B). Low-level staining in osteosarcoma tissue (C,D). High-level staining in osteosarcoma tissue (E,F).