10837-1-AP

antibody from Proteintech Group

Targeting: OPTN FIP-2, FIP2, GLC1E, HIP7, HYPL, NRP, TFIIIA-INTP

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

10837-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#10837-1-AP, RRID:AB_2156665

Product name

OPTN antibody

Antibody type

Polyclonal

Description

KD/KO validated OPTN antibody (Cat. #10837-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IHC, IP, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Panax notoginseng saponins protect PC12 cells against Aβ induced injury via promoting parkin-mediated mitophagy.

Jiang Y, Li H, Huang P, Li S, Li B, Huo L, Zhong J, Pan Z, Li Y, Xia X
Journal of ethnopharmacology 2022 Mar 1;285:114859

---

Autophagy Receptor Protein Tax1-Binding Protein 1/TRAF6-Binding Protein Is a Cellular Substrate of Enteroviral Proteinase.

Mohamud Y, Xue YC, Liu H, Ng CS, Bahreyni A, Luo H
Frontiers in microbiology 2021;12:647410

---

Hypothermia promotes mitochondrial elongation In cardiac cells via inhibition of Drp1.

Taylor D, Germano J, Song Y, Hadj-Moussa H, Marek-Iannucci S, Dhanji R, Sin J, Czer LSC, Storey KB, Gottlieb RA
Cryobiology 2021 Oct;102:42-55

---

PINK1-mediated mitophagy maintains pluripotency through optineurin.

Wang C, Liu K, Cao J, Wang L, Zhao Q, Li Z, Zhang H, Chen Q, Zhao T
Cell proliferation 2021 May;54(5):e13034

---

Proteomic analysis of aged and OPTN E50K retina in the development of normal tension glaucoma.

Liu X, Wang Q, Shao Z, Zhang S, Hou M, Jiang M, Du M, Li J, Yuan H
Human molecular genetics 2021 May 31;30(11):1030-1044

---

Coordinate regulation of the senescent state by selective autophagy.

Lee Y, Kim J, Kim MS, Kwon Y, Shin S, Yi H, Kim H, Chang MJ, Chang CB, Kang SB, Kim VN, Kim JH, Kim JS, Elledge SJ, Kang C
Developmental cell 2021 May 17;56(10):1512-1525.e7

---

Mutant HTT (huntingtin) impairs mitophagy in a cellular model of Huntington disease.

Franco-Iborra S, Plaza-Zabala A, Montpeyo M, Sebastian D, Vila M, Martinez-Vicente M
Autophagy 2021 Mar;17(3):672-689

---

TDP-43 and PINK1 mediate CHCHD10^S59L mutation-induced defects in Drosophila and in vitro.

Baek M, Choe YJ, Bannwarth S, Kim J, Maitra S, Dorn GW 2nd, Taylor JP, Paquis-Flucklinger V, Kim NC
Nature communications 2021 Mar 26;12(1):1924

---

Systematically defining selective autophagy receptor-specific cargo using autophagosome content profiling.

Zellner S, Schifferer M, Behrends C
Molecular cell 2021 Mar 18;81(6):1337-1354.e8

---

The E50K optineurin mutation impacts autophagy-mediated degradation of TDP-43 and leads to RGC apoptosis in vivo and in vitro.

Zhang S, Shao Z, Liu X, Hou M, Cheng F, Lei D, Yuan H
Cell death discovery 2021 Mar 15;7(1):49

---

Parkin-independent mitophagy via Drp1-mediated outer membrane severing and inner membrane ubiquitination.

Oshima Y, Cartier E, Boyman L, Verhoeven N, Polster BM, Huang W, Kane M, Lederer WJ, Karbowski M
The Journal of cell biology 2021 Jun 7;220(6)

---

Galectin-9 Targets NLRP3 for Autophagic Degradation to Limit Inflammation.

Wang W, Qin Y, Song H, Wang L, Jia M, Zhao C, Gong M, Zhao W
Journal of immunology (Baltimore, Md. : 1950) 2021 Jun 1;206(11):2692-2699

---

FIP200 controls the TBK1 activation threshold at SQSTM1/p62-positive condensates.

Schlütermann D, Berleth N, Deitersen J, Wallot-Hieke N, Friesen O, Wu W, Stuhldreier F, Sun Y, Berning L, Friedrich A, Mendiburo MJ, Peter C, Wiek C, Hanenberg H, Stefanski A, Stühler K, Stork B
Scientific reports 2021 Jul 5;11(1):13863

---

miR-34a-5p regulates PINK1-mediated mitophagy via multiple modes.

Tai Y, Pu M, Yuan L, Guo H, Qiao J, Lu H, Wang G, Chen J, Qi X, Tao Z, Ren J
Life sciences 2021 Jul 1;276:119415

---

The PINK1/PARK2/optineurin pathway of mitophagy is activated for protection in septic acute kidney injury.

Wang Y, Zhu J, Liu Z, Shu S, Fu Y, Liu Y, Cai J, Tang C, Liu Y, Yin X, Dong Z
Redox biology 2021 Jan;38:101767

---

Oxidative stress-induced mitophagy is suppressed by the miR-106b-93-25 cluster in a protective manner.

Zhang C, Nie P, Zhou C, Hu Y, Duan S, Gu M, Jiang D, Wang Y, Deng Z, Chen J, Chen S, Wang L
Cell death & disease 2021 Feb 24;12(2):209

---

Nitazoxanide impairs mitophagy flux through ROS-mediated mitophagy initiation and lysosomal dysfunction in bladder cancer.

Sun H, Ou T, Hu J, Yang Z, Lei Q, Li Y, Wang G, Li Y, Wu K, Wang S, Wu S
Biochemical pharmacology 2021 Aug;190:114588

---

Critical role of mitochondrial ubiquitination and the OPTN-ATG9A axis in mitophagy.

Yamano K, Kikuchi R, Kojima W, Hayashida R, Koyano F, Kawawaki J, Shoda T, Demizu Y, Naito M, Tanaka K, Matsuda N
The Journal of cell biology 2020 Sep 7;219(9)

---

Pharmacological modulation of mitochondrial calcium uniporter controls lung inflammation in cystic fibrosis.

Rimessi A, Pozzato C, Carparelli L, Rossi A, Ranucci S, De Fino I, Cigana C, Talarico A, Wieckowski MR, Ribeiro CMP, Trapella C, Rossi G, Cabrini G, Bragonzi A, Pinton P
Science advances 2020 May;6(19):eaax9093

---

UBQLN2 Promotes the Production of Type I Interferon via the TBK1-IRF3 Pathway.

Chen T, Zhang W, Huang B, Chen X, Huang C
Cells 2020 May 13;9(5)

---

Topology-dependent, bifurcated mitochondrial quality control under starvation.

Zhou Y, Long Q, Wu H, Li W, Qi J, Wu Y, Xiang G, Tang H, Yang L, Chen K, Li L, Bao F, Li H, Wang Y, Li M, Liu X
Autophagy 2020 Mar;16(3):562-574

---

Genome-wide siRNA screening reveals that DCAF4-mediated ubiquitination of optineurin stimulates autophagic degradation of Cu,Zn-superoxide dismutase.

Homma K, Takahashi H, Tsuburaya N, Naguro I, Fujisawa T, Ichijo H
The Journal of biological chemistry 2020 Mar 6;295(10):3148-3158

---

Microglia clear neuron-released α-synuclein via selective autophagy and prevent neurodegeneration.

Choi I, Zhang Y, Seegobin SP, Pruvost M, Wang Q, Purtell K, Zhang B, Yue Z
Nature communications 2020 Mar 13;11(1):1386

---

Quantitative patterns of motor cortex proteinopathy across ALS genotypes.

Nolan M, Scott C, Gamarallage MP, Lunn D, Carpenter K, McDonough E, Meyer D, Kaanumalle S, Santamaria-Pang A, Turner MR, Talbot K, Ansorge O
Acta neuropathologica communications 2020 Jul 2;8(1):98

---

Global deletion of Optineurin results in altered type I IFN signaling and abnormal bone remodeling in a model of Paget's disease.

Wong SW, Huang BW, Hu X, Ho Kim E, Kolb JP, Padilla RJ, Xue P, Wang L, Oguin TH 3rd, Miguez PA, Tseng HC, Ko CC, Martinez J
Cell death and differentiation 2020 Jan;27(1):71-84

---

The PARK10 gene USP24 is a negative regulator of autophagy and ULK1 protein stability.

Thayer JA, Awad O, Hegdekar N, Sarkar C, Tesfay H, Burt C, Zeng X, Feldman RA, Lipinski MM
Autophagy 2020 Jan;16(1):140-153

---

Lactobacillus johnsonii L531 ameliorates enteritis via elimination of damaged mitochondria and suppression of SQSTM1-dependent mitophagy in a Salmonella infantis model of piglet diarrhea.

Xia B, Yu J, He T, Liu X, Su J, Wang M, Wang J, Zhu Y
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2020 Feb;34(2):2821-2839

---

Demethylation of HACE1 gene promoter by propofol promotes autophagy of human A549 cells.

Li S, Yang H, Zhao M, Gong L, Wang Y, Lv Z, Quan Y, Wang Z
Oncology letters 2020 Dec;20(6):280

---

Loss of TAX1BP1-Directed Autophagy Results in Protein Aggregate Accumulation in the Brain.

Sarraf SA, Shah HV, Kanfer G, Pickrell AM, Holtzclaw LA, Ward ME, Youle RJ
Molecular cell 2020 Dec 3;80(5):779-795.e10

---

The Paf1 complex transcriptionally regulates the mitochondrial-anchored protein Atg32 leading to activation of mitophagy.

Zheng L, Shu WJ, Li YM, Mari M, Yan C, Wang D, Yin ZH, Jiang W, Zhou Y, Okamoto K, Reggiori F, Klionsky DJ, Song Z, Du HN
Autophagy 2020 Aug;16(8):1366-1379

---

GABARAPL2 Is Critical for Growth Restriction of Toxoplasma gondii in HeLa Cells Treated with Gamma Interferon.

Zhang Z, Gu H, Li Q, Zheng J, Cao S, Weng C, Jia H
Infection and immunity 2020 Apr 20;88(5)

---

Proteomic analysis of seminal extracellular vesicle proteins involved in asthenozoospermia by iTRAQ.

Lin Y, Liang A, He Y, Li Z, Li Z, Wang G, Sun F
Molecular reproduction and development 2019 Sep;86(9):1094-1105

---

Proteasome inhibition boosts autophagic degradation of ubiquitinated-AGR2 and enhances the antitumor efficiency of bevacizumab.

Wang D, Xu Q, Yuan Q, Jia M, Niu H, Liu X, Zhang J, Young CY, Yuan H
Oncogene 2019 May;38(18):3458-3474

---

Parkin controls brown adipose tissue plasticity in response to adaptive thermogenesis.

Cairó M, Campderrós L, Gavaldà-Navarro A, Cereijo R, Delgado-Anglés A, Quesada-López T, Giralt M, Villarroya J, Villarroya F
EMBO reports 2019 May;20(5)

---

Demethylation of the HACE1 gene promoter inhibits the proliferation of human liver cancer cells.

Yu Z, Li Y, Han T, Liu Z
Oncology letters 2019 May;17(5):4361-4368

---

STX17 dynamically regulated by Fis1 induces mitophagy via hierarchical macroautophagic mechanism.

Xian H, Yang Q, Xiao L, Shen HM, Liou YC
Nature communications 2019 May 3;10(1):2059

---

Targeting lonidamine to mitochondria mitigates lung tumorigenesis and brain metastasis.

Cheng G, Zhang Q, Pan J, Lee Y, Ouari O, Hardy M, Zielonka M, Myers CR, Zielonka J, Weh K, Chang AC, Chen G, Kresty L, Kalyanaraman B, You M
Nature communications 2019 May 17;10(1):2205

---

Association of the TBK1 mutation p.Ile334Thr with frontotemporal dementia and literature review.

Yu H, Yu W, Luo SS, Yang YJ, Liu FT, Zhang Y, Chen Y, Sun YM, Wu JJ
Molecular genetics & genomic medicine 2019 Mar;7(3):e547

---

Chronic optogenetic induction of stress granules is cytotoxic and reveals the evolution of ALS-FTD pathology.

Zhang P, Fan B, Yang P, Temirov J, Messing J, Kim HJ, Taylor JP
eLife 2019 Mar 20;8

---

Mitochondrial dysfunction and mitophagy defect triggered by heterozygous GBA mutations.

Li H, Ham A, Ma TC, Kuo SH, Kanter E, Kim D, Ko HS, Quan Y, Sardi SP, Li A, Arancio O, Kang UJ, Sulzer D, Tang G
Autophagy 2019 Jan;15(1):113-130

---

A selective inhibitor of mitofusin 1-βIIPKC association improves heart failure outcome in rats.

Ferreira JCB, Campos JC, Qvit N, Qi X, Bozi LHM, Bechara LRG, Lima VM, Queliconi BB, Disatnik MH, Dourado PMM, Kowaltowski AJ, Mochly-Rosen D
Nature communications 2019 Jan 18;10(1):329

---

The RNA-Binding Protein PUM2 Impairs Mitochondrial Dynamics and Mitophagy During Aging.

D'Amico D, Mottis A, Potenza F, Sorrentino V, Li H, Romani M, Lemos V, Schoonjans K, Zamboni N, Knott G, Schneider BL, Auwerx J
Molecular cell 2019 Feb 21;73(4):775-787.e10

---

Acteoside inhibits autophagic apoptosis of retinal ganglion cells to rescue glaucoma-induced optic atrophy.

Chen Q, Xi X, Zeng Y, He Z, Zhao J, Li Y
Journal of cellular biochemistry 2019 Aug;120(8):13133-13140

---

Activation of PINK1-Parkin-dependent mitophagy in Tri-ortho-cresyl phosphate-treated Neuro2a cells.

Wang Y, Zhang C, Shen Z, Kou R, Xie K, Song F
Chemico-biological interactions 2019 Aug 1;308:70-79

---

The cargo receptor SQSTM1 ameliorates neurofibrillary tangle pathology and spreading through selective targeting of pathological MAPT (microtubule associated protein tau).

Xu Y, Zhang S, Zheng H
Autophagy 2019 Apr;15(4):583-598

---

Parkin is a disease modifier in the mutant SOD1 mouse model of ALS.

Palomo GM, Granatiero V, Kawamata H, Konrad C, Kim M, Arreguin AJ, Zhao D, Milner TA, Manfredi G
EMBO molecular medicine 2018 Oct;10(10)

---

NDP52 interacts with mitochondrial RNA poly(A) polymerase to promote mitophagy.

Furuya N, Kakuta S, Sumiyoshi K, Ando M, Nonaka R, Suzuki A, Kazuno S, Saiki S, Hattori N
EMBO reports 2018 Dec;19(12)

---

Listeriolysin O Regulates the Expression of Optineurin, an Autophagy Adaptor That Inhibits the Growth of Listeria monocytogenes.

Puri M, La Pietra L, Mraheil MA, Lucas R, Chakraborty T, Pillich H
Toxins 2017 Sep 5;9(9)

---

Phosphorylation of Rab-coupling protein by LMTK3 controls Rab14-dependent EphA2 trafficking to promote cell:cell repulsion.

Gundry C, Marco S, Rainero E, Miller B, Dornier E, Mitchell L, Caswell PT, Campbell AD, Hogeweg A, Sansom OJ, Morton JP, Norman JC
Nature communications 2017 Mar 15;8:14646

---

Mitophagy and mitochondrial biogenesis in atrial tissue of patients undergoing heart surgery with cardiopulmonary bypass.

Andres AM, Tucker KC, Thomas A, Taylor DJ, Sengstock D, Jahania SM, Dabir R, Pourpirali S, Brown JA, Westbrook DG, Ballinger SW, Mentzer RM Jr, Gottlieb RA
JCI insight 2017 Feb 23;2(4):e89303

---

NF-κB Restricts Inflammasome Activation via Elimination of Damaged Mitochondria.

Zhong Z, Umemura A, Sanchez-Lopez E, Liang S, Shalapour S, Wong J, He F, Boassa D, Perkins G, Ali SR, McGeough MD, Ellisman MH, Seki E, Gustafsson AB, Hoffman HM, Diaz-Meco MT, Moscat J, Karin M
Cell 2016 Feb 25;164(5):896-910

---

Atg8 family LC3/GABARAP proteins are crucial for autophagosome-lysosome fusion but not autophagosome formation during PINK1/Parkin mitophagy and starvation.

Nguyen TN, Padman BS, Usher J, Oorschot V, Ramm G, Lazarou M
The Journal of cell biology 2016 Dec 19;215(6):857-874

---

Phosphatase and tensin homologue (PTEN)-induced putative kinase 1 reduces pancreatic β-cells apoptosis in glucotoxicity through activation of autophagy.

Zhang J, Chen K, Wang L, Wan X, Shrestha C, Zhou J, Mo Z
Biochemical and biophysical research communications 2016 Aug 5;476(4):299-305

---

Linear ubiquitination is involved in the pathogenesis of optineurin-associated amyotrophic lateral sclerosis.

Nakazawa S, Oikawa D, Ishii R, Ayaki T, Takahashi H, Takeda H, Ishitani R, Kamei K, Takeyoshi I, Kawakami H, Iwai K, Hatada I, Sawasaki T, Ito H, Nureki O, Tokunaga F
Nature communications 2016 Aug 24;7:12547

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Optineurin deficiency in mice is associated with increased sensitivity to Salmonella but does not affect proinflammatory NF-κB signaling.

Slowicka K, Vereecke L, Mc Guire C, Sze M, Maelfait J, Kolpe A, Saelens X, Beyaert R, van Loo G
European journal of immunology 2016 Apr;46(4):971-80

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Nucleolar stress and impaired stress granule formation contribute to C9orf72 RAN translation-induced cytotoxicity.

Tao Z, Wang H, Xia Q, Li K, Li K, Jiang X, Xu G, Wang G, Ying Z
Human molecular genetics 2015 May 1;24(9):2426-41

---

The ubiquitin kinase PINK1 recruits autophagy receptors to induce mitophagy.

Lazarou M, Sliter DA, Kane LA, Sarraf SA, Wang C, Burman JL, Sideris DP, Fogel AI, Youle RJ
Nature 2015 Aug 20;524(7565):309-314

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Oligomerization of optineurin and its oxidative stress- or E50K mutation-driven covalent cross-linking: possible relationship with glaucoma pathology.

Gao J, Ohtsubo M, Hotta Y, Minoshima S
PloS one 2014;9(7):e101206

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Interaction between optineurin and the bZIP transcription factor NRL.

Wang C, Hosono K, Ohtsubo M, Ohishi K, Gao J, Nakanishi N, Hikoya A, Sato M, Hotta Y, Minoshima S
Cell biology international 2014 Jan;38(1):16-25

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Transgenic mice with overexpression of mutated human optineurin(E50K) in the retina.

Meng Q, Xiao Z, Yuan H, Xue F, Zhu Y, Zhou X, Yang B, Sun J, Meng B, Sun X, Cheng F
Molecular biology reports 2012 Feb;39(2):1119-24

---

Optineurin in neurodegenerative diseases.

Osawa T, Mizuno Y, Fujita Y, Takatama M, Nakazato Y, Okamoto K
Neuropathology : official journal of the Japanese Society of Neuropathology 2011 Dec;31(6):569-74

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p62 positive, TDP-43 negative, neuronal cytoplasmic and intranuclear inclusions in the cerebellum and hippocampus define the pathology of C9orf72-linked FTLD and MND/ALS.

Al-Sarraj S, King A, Troakes C, Smith B, Maekawa S, Bodi I, Rogelj B, Al-Chalabi A, Hortobágyi T, Shaw CE
Acta neuropathologica 2011 Dec;122(6):691-702

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 10837-1-AP(OPTN antibody) at dilution of 1:800

Sample type

cell line

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of MCF-7 cells, using OPTN antibody 10837-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Sample type

cell line

Immunoprecipitation

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

IP Result of anti-OPTN (IP:10837-1-AP, 3ug; Detection:10837-1-AP 1:800) with mouse brain tissue lysate 8000ug.

Sample type

tissue

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human pancreas cancer using 10837-1-AP(OPTN antibody) at dilution of 1:50 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human pancreas cancer using 10837-1-AP(OPTN antibody) at dilution of 1:50 (under 40x lens)

Sample type

tissue