PAB29391

antibody from Abnova Corporation

Targeting: OPTN FIP-2, FIP2, GLC1E, HIP7, HYPL, NRP, TFIIIA-INTP

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB29391

Provider

Abnova Corporation

Product name

OPTN polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant human OPTN.

Antigen sequence

LELAEKALASKQLQMDEMKQTIAKQEEDLETMTIL
RAQMEVYCSDFHAERAAREKIHEEKEQLALQLAVL
LKENDAFEDGGRQSLMEMQSRHGARTSDSDQQAYL
VQRGAEDRDWRQQRNIPIHSCPKCGEVL


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Human cell line RT-4 Lane 2: Human cell line U-251MG sp Lane 3: Human plasma (IgG/HSA depleted) Lane 4: Human liver tissue Lane 5: Human tonsil tissue with OPTN polyclonal antibody (Cat # PAB29391) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A-431 with OPTN polyclonal antibody (Cat # PAB29391) at 1-4 ug/mL concentration shows positivity in cytoplasm.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human placenta with OPTN polyclonal antibody (Cat # PAB29391) shows strong cytoplasmic positivity in trophoblastic cells at 1:200-1:500 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)