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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [14]
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- Validations
- Immunohistochemistry [2]
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- Product number
- NB300-561 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB300-561, RRID:AB_10129120
- Product name
- Mouse Monoclonal Prolactin R Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human, Mouse, Rat, Bovine, Hamster, Porcine, Rabbit, Sheep
- Host
- Mouse
- Antigen sequence
This antibody has been shown to rea
ct with the extracellular portion o
f the receptor, but not in the liga
nd binding domain.- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Controlled synchronization of prolactin/STAT5 and AKT1/mTOR in bovine mammary epithelial cells.
Laminin-dependent integrin β1 signaling regulates milk protein synthesis via prolactin/STAT5 pathway in bovine mammary epithelial cells.
Short-Term Hyperprolactinemia Reduces the Expression of Purinergic P2X7 Receptors during Allergic Inflammatory Response of the Lungs.
Internalization of prolactin receptor and prolactin in transfected cells does not involve nuclear translocation.
Prolactin receptors and JAK2 in islets of Langerhans: an immunohistochemical analysis.
The prolactin receptor in the fetal rat: cellular localization of messenger ribonucleic acid, immunoreactive protein, and ligand-binding activity and induction of expression in late gestation.
JAK2 activation and cell proliferation induced by antibody-mediated prolactin receptor dimerization.
Prolactin receptor expression in human hematopoietic tissues analyzed by flow cytofluorometry.
Expression of prolactin receptors in murine lymphoid cells in normal and autoimmune situations.
Mutational analysis of the ligand-binding domain of the prolactin receptor.
Identification and functional activity of prolactin receptors in thymic epithelial cells.
Identification and functional activity of prolactin receptors in thymic epithelial cells.
Characterization and applications of monoclonal antibodies to the prolactin receptor.
Hepatic prolactin receptors in the rat: characterization using monoclonal antireceptor antibodies.
Wang B, Shi L, Men J, Li Q, Hou X, Wang C, Zhao F
In vitro cellular & developmental biology. Animal 2020 Mar;56(3):243-252
In vitro cellular & developmental biology. Animal 2020 Mar;56(3):243-252
Laminin-dependent integrin β1 signaling regulates milk protein synthesis via prolactin/STAT5 pathway in bovine mammary epithelial cells.
Wang B, Men J, Wang C, Hou X, Zhao F
Biochemical and biophysical research communications 2020 Apr 2;524(2):288-294
Biochemical and biophysical research communications 2020 Apr 2;524(2):288-294
Short-Term Hyperprolactinemia Reduces the Expression of Purinergic P2X7 Receptors during Allergic Inflammatory Response of the Lungs.
Ochoa-Amaya JE, Queiroz-Hazarbassanov N, Namazu LB, Calefi AS, Tobaruela CN, Margatho R, Palermo-Neto J, Ligeiro de Oliveira AP, Felicio LF
Neuroimmunomodulation 2018;25(1):34-41
Neuroimmunomodulation 2018;25(1):34-41
Internalization of prolactin receptor and prolactin in transfected cells does not involve nuclear translocation.
Perrot-Applanat M, Gualillo O, Buteau H, Edery M, Kelly PA
Journal of cell science 1997 May;110 ( Pt 9):1123-32
Journal of cell science 1997 May;110 ( Pt 9):1123-32
Prolactin receptors and JAK2 in islets of Langerhans: an immunohistochemical analysis.
Sorenson RL, Stout LE
Endocrinology 1995 Sep;136(9):4092-8
Endocrinology 1995 Sep;136(9):4092-8
The prolactin receptor in the fetal rat: cellular localization of messenger ribonucleic acid, immunoreactive protein, and ligand-binding activity and induction of expression in late gestation.
Royster M, Driscoll P, Kelly PA, Freemark M
Endocrinology 1995 Sep;136(9):3892-900
Endocrinology 1995 Sep;136(9):3892-900
JAK2 activation and cell proliferation induced by antibody-mediated prolactin receptor dimerization.
Rui H, Lebrun JJ, Kirken RA, Kelly PA, Farrar WL
Endocrinology 1994 Oct;135(4):1299-306
Endocrinology 1994 Oct;135(4):1299-306
Prolactin receptor expression in human hematopoietic tissues analyzed by flow cytofluorometry.
Dardenne M, de Moraes Mdo C, Kelly PA, Gagnerault MC
Endocrinology 1994 May;134(5):2108-14
Endocrinology 1994 May;134(5):2108-14
Expression of prolactin receptors in murine lymphoid cells in normal and autoimmune situations.
Gagnerault MC, Touraine P, Savino W, Kelly PA, Dardenne M
Journal of immunology (Baltimore, Md. : 1950) 1993 Jun 15;150(12):5673-81
Journal of immunology (Baltimore, Md. : 1950) 1993 Jun 15;150(12):5673-81
Mutational analysis of the ligand-binding domain of the prolactin receptor.
Rozakis-Adcock M, Kelly PA
The Journal of biological chemistry 1991 Sep 5;266(25):16472-7
The Journal of biological chemistry 1991 Sep 5;266(25):16472-7
Identification and functional activity of prolactin receptors in thymic epithelial cells.
Dardenne M, Kelly PA, Bach JF, Savino W
Proceedings of the National Academy of Sciences of the United States of America 1991 Nov 1;88(21):9700-4
Proceedings of the National Academy of Sciences of the United States of America 1991 Nov 1;88(21):9700-4
Identification and functional activity of prolactin receptors in thymic epithelial cells.
Dardenne M, Kelly PA, Bach JF, Savino W
Proceedings of the National Academy of Sciences of the United States of America 1991 Nov 1;88(21):9700-4
Proceedings of the National Academy of Sciences of the United States of America 1991 Nov 1;88(21):9700-4
Characterization and applications of monoclonal antibodies to the prolactin receptor.
Okamura H, Zachwieja J, Raguet S, Kelly PA
Endocrinology 1989 May;124(5):2499-508
Endocrinology 1989 May;124(5):2499-508
Hepatic prolactin receptors in the rat: characterization using monoclonal antireceptor antibodies.
Katoh M, Raguet S, Zachwieja J, Djiane J, Kelly PA
Endocrinology 1987 Feb;120(2):739-49
Endocrinology 1987 Feb;120(2):739-49
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: Prolactin R Antibody (U5) [NB300-561] - Mammary gland tissue from lactating light horse mare (equine) was incubated in anti-prolactin receptor monoclonal mouse antibody at 1:100 for 1 hour at 23C and for 24 hours at 4C. Second antibody was biotinylated goat anti-mouse IgG at 23C for 1 hour. Development using avidin-biotin complex 23C for 1 hour plus 3,3-diaminobenzadine for 15 min and counterstained with haematoxylin.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: Prolactin R Antibody (U5) [NB300-561] - Figure 1 shows immunohistochemical staining of PRL receptor in rat Islets of Langerhans using NB300-561.
