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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- PAB15575 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB15575, RRID:AB_10682875
- Product name
- RAD18 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against partial recombinant RAD18.
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Differential regulation of Rad18 through Rad6-dependent mono- and polyubiquitination.
Rad18 guides poleta to replication stalling sites through physical interaction and PCNA monoubiquitination.
Dysfunction of human Rad18 results in defective postreplication repair and hypersensitivity to multiple mutagens.
Miyase S, Tateishi S, Watanabe K, Tomita K, Suzuki K, Inoue H, Yamaizumi M
The Journal of biological chemistry 2005 Jan 7;280(1):515-24
The Journal of biological chemistry 2005 Jan 7;280(1):515-24
Rad18 guides poleta to replication stalling sites through physical interaction and PCNA monoubiquitination.
Watanabe K, Tateishi S, Kawasuji M, Tsurimoto T, Inoue H, Yamaizumi M
The EMBO journal 2004 Oct 1;23(19):3886-96
The EMBO journal 2004 Oct 1;23(19):3886-96
Dysfunction of human Rad18 results in defective postreplication repair and hypersensitivity to multiple mutagens.
Tateishi S, Sakuraba Y, Masuyama S, Inoue H, Yamaizumi M
Proceedings of the National Academy of Sciences of the United States of America 2000 Jul 5;97(14):7927-32
Proceedings of the National Academy of Sciences of the United States of America 2000 Jul 5;97(14):7927-32
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Identification of RAD18 protein in crude extract of A-549 cells by western blotting with RAD18 polyclonal antibody (Cat # PAB15575). The lower thick band is native RAD18 and the upper thin band is mono-ubiquitinated RAD18 protein.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Indirect immunofluorescence staining of RAD18 protein in GM637 cells by RAD18 polyclonal antibody (Cat # PAB15575). RAD18 protein is stained as yellow dots in nuclei.
- Validation comment
- Immunofluorescence
