AF4474

antibody from R&D Systems

Targeting: CD2AP CMS

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AF4474

Provider

R&D Systems

Product name

Human/Mouse/Rat CD2AP Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects endogenous human, mouse, and rat CD2AP in Western blots.

Reactivity

Human, Mouse, Rat

Host

Sheep

Conjugate

Unconjugated

Antigen sequence

Q9Y5K6


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Visualisation studies and glomerular filtration in early diabetic rats.

Nakamoto H
Journal of biomechanics 2017 Jan 4;50:138-143

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CIN85 is required for Cbl-mediated regulation of antigen receptor signaling in human B cells.

Niiro H, Jabbarzadeh-Tabrizi S, Kikushige Y, Shima T, Noda K, Ota S, Tsuzuki H, Inoue Y, Arinobu Y, Iwasaki H, Shimoda S, Baba E, Tsukamoto H, Horiuchi T, Taniyama T, Akashi K
Blood 2012 Mar 8;119(10):2263-73

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human/Mouse/Rat CD2AP by Western Blot. Western blot shows lysates of MOLT-4 human acute lymphoblastic leukemia cell line, Ramos human Burkitt's lymphoma cell line, KG-1 human acute myelogenous leukemia cell line, M1 mouse myeloid leukemia cell line, and Y3-Ag rat myeloid cell line. PVDF membrane was probed with 0.2 µg/mL of Human/Mouse/Rat CD2AP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4474) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD2AP at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.