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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- ANC-002-200UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Nicotinic Acetylcholine Receptor alpha 2 (CHRNA2) (extracellular) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human SH-SY5Y neuroblastoma cell lysate: - 1. Anti-Nicotinic Acetylcholine Receptor alpha 2 (CHRNA2) (extracellular) Antibody (#ANC-002), (1:200). 2. Anti-Nicotinic Acetylcholine Receptor alpha 2 (CHRNA2) (extracellular) Antibody , preincubated with Nicotinic Acetylcholine Receptor alpha 2/CHRNA2 (extracellular) Blocking Peptide (#BLP-NC002).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human SH-SY5Y neuroblastoma cell lysate: - 1. Anti-Nicotinic Acetylcholine Receptor alpha 2 (CHRNA2) (extracellular) Antibody (#ANC-002), (1:200). 2. Anti-Nicotinic Acetylcholine Receptor alpha 2 (CHRNA2) (extracellular) Antibody , preincubated with Nicotinic Acetylcholine Receptor alpha 2/CHRNA2 (extracellular) Blocking Peptide (#BLP-NC002).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain membranes: - 1,2. Anti-Nicotinic Acetylcholine Receptor alpha 2 (CHRNA2) (extracellular) Antibody (#ANC-002), (1:400).3,4. Anti-Nicotinic Acetylcholine Receptor alpha 2 (CHRNA2) (extracellular) Antibody , preincubated with Nicotinic Acetylcholine Receptor alpha 2/CHRNA2 (extracellular) Blocking Peptide (#BLP-NC002).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of nAChR alpha 2 in rat PC12 pheochromocytoma cells - Cell surface detection of nAChR alpha 2 in live intact rat PC12 pheochromocytoma cells. A. Extracellular staining of live cells with Anti-Nicotinic Acetylcholine Receptor alpha 2 (CHRNA2) (extracellular) Antibody (#ANC-002), (1:50), followed by goat Anti-rabbit-AlexaFluor-594 (red). B. Live image of the cells. C. Merge of the two images.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of nAChR alpha 2 inrat deep cerebellar nucleus - Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-Nicotinic Acetylcholine Receptor alpha 2 (CHRNA2) (extracellular) Antibody (#ANC-002), (1:100). Staining reveals expression of nAChR alpha 2 (red) in cells with neuronal outline (arrows point at some examples) in the red nucleus.DAPI is used as thecounterstain (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of nAChR alpha 2 inrat deep cerebellar nucleus - Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-Nicotinic Acetylcholine Receptor alpha 2 (CHRNA2) (extracellular) Antibody (#ANC-002), (1:100). Staining reveals expression of nAChR alpha 2 (red) in cells with neuronal outline (arrows point at some examples) in the red nucleus.DAPI is used as thecounterstain (blue).
