H00008812-M01

antibody from Abnova Corporation

Targeting: CCNK CPR4

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00008812-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00008812-M01, RRID:AB_425784

Product name

CCNK monoclonal antibody (M01), clone 3B7-1B9

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant CCNK.

Antigen sequence

MKENKENSSPSVTSANLDHTKPCWYWDKKDLAHTP
SQLEGLDPATEARYRREGARFIFDVGTRLGLHYDT
LATGIIYFHRFYMFHSFKQFPRYVTGACCLFLAGK
VEETPKKCKDIIKTARSLLNDVQFGQFGDDPKEEV
MVLERILLQTIKFDLQVEHPYQFLLKYAKQLKGDK
NKIQKLVQMAWTFVNDSLCTTLSLQWEPEIIAVAV
MYLAGRLCKFEIQEWTSKPMYRRWWEQFVQDVPVD
VLEDICHQILDLYSQGKQQMPHHTPHQLQQPPSLQ
PTPQVPQVQQSQPSQSSEPSQPQQKDPLILLQGWA
CRQPATHLLPSPLEDSLLCPRPFPHPACLQLGGWG
GQPG


Isotype

IgG

Antibody clone number

3B7-1B9

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References

Submitted references

The human HECA interacts with cyclins and CDKs to antagonize Wnt-mediated proliferation and chemoresistance of head and neck cancer cells.

Dowejko A, Bauer R, Bauer K, Müller-Richter UD, Reichert TE
Experimental cell research 2012 Mar 10;318(5):489-99

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

CCNK monoclonal antibody (M01), clone 3B7-1B9. Western Blot analysis of CCNK expression in PRC/PRF/5.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of CCNK expression in transfected 293T cell line by CCNK monoclonal antibody (M01), clone 3B7-1B9.Lane 1: CCNK transfected lysate(41 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged CCNK is approximately 0.03ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol