Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- ELISA [1]
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Validation data
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- Product number
- H00008812-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00008812-M01, RRID:AB_2073607
- Product name
- Mouse Monoclonal CCNK Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. CCNK - cyclin K
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references The human HECA interacts with cyclins and CDKs to antagonize Wnt-mediated proliferation and chemoresistance of head and neck cancer cells.
Dowejko A, Bauer R, Bauer K, Müller-Richter UD, Reichert TE
Experimental cell research 2012 Mar 10;318(5):489-99
Experimental cell research 2012 Mar 10;318(5):489-99
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CCNK Antibody (3B7-1B9) [H00008812-M01] - Analysis of CCNK over-expressed 293 cell line, cotransfected with CCNK Validated Chimera RNAi ( Cat # H00008812-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CCNK monoclonal antibody (M01), clone 3B7-1B9 (Cat # H00008812-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CCNK Antibody (3B7-1B9) [H00008812-M01] - Analysis of CCNK expression in transfected 293T cell line by CCNK monoclonal antibody (M01), clone 3B7-1B9.Lane 1: CCNK transfected lysate(41 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CCNK Antibody (3B7-1B9) [H00008812-M01] - CCNK monoclonal antibody (M01), clone 3B7-1B9. Analysis of CCNK expression in PRC/PRF/5.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: CCNK Antibody (3B7-1B9) [H00008812-M01] - Detection limit for recombinant GST tagged CCNK is approximately 0.03ng/ml as a capture antibody.