Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [5]
- Immunohistochemistry [2]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- A301-939A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Cyclin K Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- The recommended shelf life for this product is 1 year from date of receipt.
- Concentration
- 1 mg/mL
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human Cyclin K by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, MCF-7, Jurkat, HeLa, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Cyclin K antibody (Product # A301-939A lot 9) used for WB at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 75 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human Cyclin K by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, MCF-7, Jurkat, HeLa, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Cyclin K antibody (Product # A301-939A lot 9) used for WB at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 75 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of mouse Cyclin K by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3, CH27, TCMK-1, BW5147.3, and CT26 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Cyclin K antibody (Product # A301-939A lot 9) used for WB at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human Cyclin K by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Cyclin K antibody A301-939A (lot A301-939A-5) used for IP at 3 µg per reaction. Cyclin K was also immunoprecipitated by rabbit anti-Cyclin K antibody BL6428. For blotting immunoprecipitated Cyclin K, A301-939A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human Cyclin K by western blot. Samples: Whole cell lysate (50 µg) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Cyclin K antibody A301-939A (lot A301-939A-5) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human Cyclin K by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-Cyclin K (Product # A301-939A; lot 9) used at a dilution of 1:5,000 (0.2 µg/mL). Detection: DAB.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human Cyclin K by immunohistochemistry. Sample: FFPE section of human head and neck squamous cell carcinoma. Antibody: Affinity purified rabbit anti-Cyclin K (Cat. No. A301-939A Lot 5) used at a dilution of 1:5,000 (0.2µg/ml). Detection: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human Cyclin K by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-Cyclin K antibody (Product # A301-939A lot 9) used for IP at 6 µg per reaction. Cyclin K was also immunoprecipitated by a previous lot of this antibody (A301-939A lot 8) and rabbit anti Cyclin-K antibody (BL6428). For blotting immunoprecipitated Cyclin K (Product # A301-939A) was used at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 10 seconds.
