PA5-117675

antibody from Invitrogen Antibodies

Targeting: CD300LG CLM9, Trem4

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-117675

Provider

Invitrogen Antibodies

Product name

CD300g Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

This antibody has been tested in direct-ELISA

Reactivity

Human, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

2.983 mg/mL

Storage

-20°C or -80°C if preferred

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of CD300g using CD300g Polyclonal Antibody (Product # PA5-117675) at 6 µg/mL. Positive WB detected in: Rat kidney tissue. Secondary: Goat polyclonal to rabbit IgG at 1:50,000 dilution. Predicted band size: 37, 27, 25, 34, 30 kDa. Observed band size: 37 kDa

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence analysis of CD300g in 293 cells using CD300g Polyclonal Antibody (Product # PA5-117675) diluted at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence analysis of CD300g in 293 cells using CD300g Polyclonal Antibody (Product # PA5-117675) diluted at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immnohistochemistry (Paraffin) analysis of CD300g in paraffin-embedded human placenta tissue using CD300g Polyclonal Antibody (Product # PA5-117675) diluted at 1:200. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.