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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-31257 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HAUS6 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: Jurkat, 293T, A431, HeLa.
- Concentration
- 1.0 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HAUS6 using Various whole cell extracts (30 µg). Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a HAUS6 polyclonal antibody (Product # PA5-31257) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP-WB assay to show that Hice1 co-immunoprecipitated with other Augmin components: HAUS6 (Product # PA5-31257), HAUS2 (Product # PA5-31258) and HAUS1 (Product # PA5-34874) in U2OS cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of HAUS6 was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a HAUS6 Polyclonal Antibody (Product # PA5-31257) at a dilution of 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using HAUS6 Polyclonal Antibody (Product # PA5-31257). Sample (30 µg of whole cell lysate). Lane A: JurKat. 7.5% SDS PAGE. HAUS6 Polyclonal Antibody (Product # PA5-31257) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HAUS6 Immunofluorescence using HAUS6 Polyclonal Antibody (Product # PA5-31257) at 1: 500; red) co-localizes at the mitotic spindle with Hice1-GFP (HAUS-GFP; green) in U2OS cells. DAPI staining (blue) shows mitotic chromosomes. Arrows indicate spindle poles.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HAUS6 Polyclonal Antibody detects HAUS6 protein on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. HAUS6 Polyclonal Antibody (Product # PA5-31257) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
