18065-1-AP

antibody from Proteintech Group

Targeting: ALDOB

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

18065-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#18065-1-AP, RRID:AB_2273968

Product name

ALDOB antibody

Antibody type

Polyclonal

Description

KD/KO validated ALDOB antibody (Cat. #18065-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IHC, IP, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Multi-omics profiling of living human pancreatic islet donors reveals heterogeneous beta cell trajectories towards type 2 diabetes.

Wigger L, Barovic M, Brunner AD, Marzetta F, Schöniger E, Mehl F, Kipke N, Friedland D, Burdet F, Kessler C, Lesche M, Thorens B, Bonifacio E, Legido-Quigley C, Barbier Saint Hilaire P, Delerive P, Dahl A, Klose C, Gerl MJ, Simons K, Aust D, Weitz J, Distler M, Schulte AM, Mann M, Ibberson M, Solimena M
Nature metabolism 2021 Jul;3(7):1017-1031

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Single-Cell Transcriptome Analysis Reveals Intratumoral Heterogeneity in ccRCC, which Results in Different Clinical Outcomes.

Hu J, Chen Z, Bao L, Zhou L, Hou Y, Liu L, Xiong M, Zhang Y, Wang B, Tao Z, Chen K
Molecular therapy : the journal of the American Society of Gene Therapy 2020 Jul 8;28(7):1658-1672

---

Measurement of Organ-Specific and Acute-Phase Blood Protein Levels in Early Lyme Disease.

Zhou Y, Qin S, Sun M, Tang L, Yan X, Kim TK, Caballero J, Glusman G, Brunkow ME, Soloski MJ, Rebman AW, Scavarda C, Cooper D, Omenn GS, Moritz RL, Wormser GP, Price ND, Aucott JN, Hood L
Journal of proteome research 2020 Jan 3;19(1):346-359

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Loss of hepatic aldolase B activates Akt and promotes hepatocellular carcinogenesis by destabilizing the Aldob/Akt/PP2A protein complex.

He X, Li M, Yu H, Liu G, Wang N, Yin C, Tu Q, Narla G, Tao Y, Cheng S, Yin H
PLoS biology 2020 Dec;18(12):e3000803

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Plasma Protein Comparison between Dairy Cows with Inactive Ovaries and Estrus.

Zhao C, Shu S, Bai Y, Wang D, Xia C, Xu C
Scientific reports 2019 Sep 23;9(1):13709

---

Accumulation of fructose 1,6-bisphosphate protects clear cell renal cell carcinoma from oxidative stress.

Wang J, Wu Q, Qiu J
Laboratory investigation; a journal of technical methods and pathology 2019 Jun;99(6):898-908

---

SIRT5-mediated SDHA desuccinylation promotes clear cell renal cell carcinoma tumorigenesis.

Ma Y, Qi Y, Wang L, Zheng Z, Zhang Y, Zheng J
Free radical biology & medicine 2019 Apr;134:458-467

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The Expression of Aldolase B in Islets Is Negatively Associated With Insulin Secretion in Humans.

Gerst F, Jaghutriz BA, Staiger H, Schulte AM, Lorza-Gil E, Kaiser G, Panse M, Haug S, Heni M, Schütz M, Stadion M, Schürmann A, Marzetta F, Ibberson M, Sipos B, Fend F, Fleming T, Nawroth PP, Königsrainer A, Nadalin S, Wagner S, Peter A, Fritsche A, Richter D, Solimena M, Häring HU, Ullrich S, Wagner R
The Journal of clinical endocrinology and metabolism 2018 Dec 1;103(12):4373-4383

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Proteomics and metabolomics analysis of hepatic mitochondrial metabolism in alcohol-preferring and non-preferring rats.

Zeng HL, Yang Q, Du H, Li H, Shen Y, Liu T, Chen X, Kamal GM, Guan Q, Cheng L, Wang J, Xu F
Oncotarget 2017 Nov 24;8(60):102020-102032

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CUE domain-containing protein 2 promotes the Warburg effect and tumorigenesis.

Zhong X, Tian S, Zhang X, Diao X, Dong F, Yang J, Li Z, Sun L, Wang L, He X, Wu G, Hu X, Wang L, Song L, Zhang H, Pan X, Li A, Gao P
EMBO reports 2017 May;18(5):809-825

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Fructose-1,6-bisphosphate and aldolase mediate glucose sensing by AMPK.

Zhang CS, Hawley SA, Zong Y, Li M, Wang Z, Gray A, Ma T, Cui J, Feng JW, Zhu M, Wu YQ, Li TY, Ye Z, Lin SY, Yin H, Piao HL, Hardie DG, Lin SC
Nature 2017 Aug 3;548(7665):112-116

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Aldolase B inhibits metastasis through Ten-Eleven Translocation 1 and serves as a prognostic biomarker in hepatocellular carcinoma.

Tao QF, Yuan SX, Yang F, Yang S, Yang Y, Yuan JH, Wang ZG, Xu QG, Lin KY, Cai J, Yu J, Huang WL, Teng XL, Zhou CC, Wang F, Sun SH, Zhou WP
Molecular cancer 2015 Sep 17;14:170

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cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions.

Sun L, Song L, Wan Q, Wu G, Li X, Wang Y, Wang J, Liu Z, Zhong X, He X, Shen S, Pan X, Li A, Wang Y, Gao P, Tang H, Zhang H
Cell research 2015 Apr;25(4):429-44

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

mouse kidney tissue were subjected to SDS PAGE followed by western blot with 18065-1-AP(ALDOB antibody) at dilution of 1:4000

Sample type

tissue

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using ALDOB antibody 18065-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Sample type

cell line

Immunoprecipitation

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

IP Result of anti-ALDOB (IP:18065-1-AP, 4ug; Detection:18065-1-AP 1:1000) with mouse kidney tissue lysate 4000ug.

Sample type

tissue

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human liver using 18065-1-AP(ALDOB antibody) at dilution of 1:100 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human liver using 18065-1-AP(ALDOB antibody) at dilution of 1:100 (under 40x lens)

Sample type

tissue