Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [5]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX101363 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX101363, RRID:AB_11169399
- Product name
- Aldolase B antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Characterization of fructose-1,6-bisphosphate aldolase during anoxia in the tolerant turtle, Trachemys scripta elegans: an assessment of enzyme activity, expression and structure.
Dawson NJ, Biggar KK, Storey KB
PloS one 2013;8(7):e68830
PloS one 2013;8(7):e68830
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Supportive validation
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- Experimental details
- Sample (30 ?g of whole cell lysate) A: 293T B: A431 C: HeLa 10% SDS PAGE GTX101363 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Aldolase B antibody detects ALDOB protein by western blot analysis.A. 30 ?g Neuro2A whole cell lysate/extract B. 30 ?g GL261 whole cell lysate/extractC. 30 ?g C8D30 whole cell lysate/extract10% SDS-PAGEAldolase B antibody (GTX101363) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Aldolase B antibody detects ALDOB protein by western blot analysis.A. 30 ?g PC-12 whole cell lysate/extract10% SDS-PAGEAldolase B antibody (GTX101363) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with Aldolase B antibody (GTX101363) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various tissue extracts (50 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with Aldolase B antibody (GTX101363) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of methanol-fixed HeLa, using Aldolase B(GTX101363) antibody at 1:500 dilution.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Aldolase B antibody detects ALDOB protein at cytosol on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma tissue. Aldolase B antibody (GTX101363) dilution: 1:500.